强迫症大鼠模型敏感脑区代谢物的磁共振波谱动态分析

Dynamic study of magnetic resonance spectrum about sensitive brain structure of rat obsessive compulsive disorder model

目的:运用磁共振氢质子波谱(1H-MRS)技术对多巴胺激动剂喹吡罗强迫症模型大鼠的海马、额叶组织代谢物进行动态定量分析。方法:将40只行为学评分相近的SD大鼠随机分成对照组(10只)及喹吡罗组(30只),喹吡罗组大鼠每周进行2次喹吡罗颈部皮下注射,对照组给予同等剂量的生理盐水颈部皮下注射,共给药10次;每次给药后对每只大鼠进行3.0 T超高场强MRI扫描及磁共振质子波谱检测,检测指标包括N-乙酰天门冬氨酸(NAA)、肌酸(Cr)、胆碱复合物(Cho)及肌醇(MI),分别计算NAA、Cho、MI与Cr的比值。结果:1与对照组比较,喹吡罗组大鼠双侧海马区NAA/Cr自建模第10天起明显减低,自第24天起明显升高,P均<0.05;双侧额叶区NAA/Cr从第10天开始较对照组明显升高(P<0.05),第21-34天维持在第21天的水平左右。2喹吡罗组大鼠双侧额叶区Cho/Cr值自第21天起较对照组明显升高(P<0.05);而海马区Cho/Cr与对照组比较未见明显变化(P>0.05)。3喹吡罗组大鼠双侧海马区MI/Cr值从第7天开始较对照组明显减低(P<0.05),第21-34天维持在第21天水平左右;双侧额叶区MI/Cr值较对照组未见显著变化(P>0.05)。结论:喹吡罗组大鼠脑内多个代谢物指标存在异常,且部分呈现一定的规律性。

Objective: To analyze the metabolism of the hippocampus and frontal lobe tissue of the rat obsessive compulsive disorder model by1H-MRS. Methods: A total of 40 SD rats were randomly divided into control group and quinpirole group,10 rats in control group,30 rats in quinpirole group. The rats in quinpirole group received quinpirole subcutaneous injection twice a week,the control group received the same amount of saline injection. All of the rats were injected ten times. Every time after delivery,all of the rats received 3. 0T MRI and analyzing technology1H-MRS( indexes including NAA,Cr,Cho and MI). Results: 1 Compared with the control group,the values of NAA / Cr of the bilateral hippocampus of the quinpirole group were significantly reduced from the 10thday( P < 0. 05),and the values began to significantly rise from the 24thday( P < 0. 05); the values of NAA / Cr of the bilateral frontal lobe of the quinpirole group were significantly rose from the 10thday( P < 0. 05),and keep the same level on the 21-34 thday. 2 The values of Cho / Cr of the bilateral frontal lobe of the quinpirole group were significantly rose from the 21thday( P < 0. 05); but the values of hippocampus had no obvious change( P > 0. 05). 3 The values MI/Cr of the bilateral hippocampus of the quinpirole group were significantly reduced from the 7thday,and keep the same level on the 21-34thday( P < 0. 05); but the values of frontal lobe had no obvious change( P >0. 05). Conclusion: Many metabolite indexes were abnormal in quinpirole rat model,and some showed certain regularity.