髓系细胞触发受体-1对大鼠肠巨噬细胞氧化应激的影响

Effect of triggering receptor expressed on myeloid cells-1 expression on the oxidative stress of intestinal macrophages in rats

目的:探讨髓系细胞触发受体(triggering receptor expressed on myeloid cells,TREM)-1的表达对大鼠肠巨噬细胞氧化应激的影响。方法:体外培养肠巨噬细胞,随机分3组,对照组常规培养,脂多糖组和脂多糖+LP17组分别于培养基中加入脂多糖(1 mg/L),脂多糖(1 mg/L)+LP17(0.1 mg/L),培养6 h,反转录-聚合酶链反应(reverse transcription-PCR,RT-PCR)和蛋白质印迹法检测各组细胞TREM-1及TNF-α的表达,活性氧荧光探针(DHE)检测细胞活性氧含量的变化。结果:与对照组比较,脂多糖组和脂多糖+LP17组肠巨噬细胞TREM-1及TNF-α的基因及蛋白表达明显增加(P均<0.05),且脂多糖+LP17组明显低于脂多糖组(P<0.05)。与对照组比较,脂多糖组和脂多糖+LP17组活性氧含量明显增加,且脂多糖组高于脂多糖+LP17组。结论:抑制肠巨噬细胞TREM-1的表达可减轻氧化应激对肠黏膜屏障的损伤。

Objective:To investigate the effect of triggering receptor expressed on myeloid cells-1 (TREM-1)expression on the oxidative stress of rat intestinal macrophages.Methods:Intestinal macropha-ges were cultured in vitro,and randomly divided into three groups,the control group was routinely cultured, LPS group and LPS +LP17 groups were added lipopolysaccharide (1 mg/L),lipopolysaccharide (1 mg/L)+LP17 (0.1 mg/L)in the medium,respectively.The expression levels of TREM-1 and TNF-αwere deter-mined by reverse transcription-PCR(RT-PCR)and Western blotting method;and the levels of reactive ox-gen species(ROS)was detected by ROS Fluorescent Probe-Dihyclroethidium(DHE)method in each group. Results:Compared with the control group,the mRNA and protein expression levels of TREM-1 and TNF-αin LPS group and LPS +LP17 group were significantly increased (both P <0.05),meanwhile compared with the LPS group,the mRNA and protein expression levels of TREM-1 and TNF-αwas dramatic lower than in LPS +LP17 group (P >0.05).Compared with the control group,the active oxygen species remarkably in-creased in LPS group and LPS +LP17 group,at the same time the active oxygen species in LPS group was significantly higher than LPS +LP17 group.Conclusion:Inhibiting the expression of TREM-1 in intestinal macrophages could reduce the damage induced by oxidative stress on intestinal mucosal barrier.