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囊性纤维跨膜转导调控因子的基因克隆及在小鼠组织中的表达 被引量：1

Cystic fibrosis transmembrane conductance regulator gene expression in mouse tissues
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摘要 目的 :观察囊性纤维跨膜转导调控因子 (CFTCR)在小鼠不同组织中的表达及定位。方法 :采用RT -PCR的方法 ,设计两对引物 ,位于核苷酸第 1348～ 170 8之间 ,另一对引物为 β -actine,用来检测RNA是否完整。然后用抗CFTCR抗体 ,应用免疫组化方法检测其在牙胚组织中的定位。结果 :CFTCR在小鼠的14种组织中有表达 ,包括牙胚组织和骨组织。并且CFTCR在牙成釉细胞中高表达。结论 :本研究首次发现CFTCR在矿化的组织中包括牙齿的成釉细胞中高表达 。 AIM:To characterize (cystic fibrosis transmembrane conductance regulator,CFTCR) gene expression in different murine tissues and further verify localization of CFTCR.METHODS:CFTCR expression was evaluated by RT-PCR with primers spanning in the region between nucleotides 1 348 and 1 708.β-actin primers were used to assess the integrity of the RNA.Antibodies against the CFTCR were used to localize CFTCR in the tooth of mouse. RESULTS: CFTCR expression was seen in all 14 tissues examined.Positive staining for CFTCR was detected in ameloblast.CONCLUSION:This study shows that the CFTCR is ubiquitously expression in murine tissues and ameloblast.This is the first report that the CFTCR is expressed in mineralized tissues including tooth. Expression of CFTCR in tooth indicates that this ion channel may be important in the mineralization process of enamel.
作者 隋文 Boyd C Wreht JT
机构地区 第四军医大学口腔医学院 DepartmentofOralScience
出处 《牙体牙髓牙周病学杂志》 CAS 2003年第6期295-300,T001,共7页 Chinese Journal of Conservative Dentistry
关键词 小鼠 CFTCR 牙釉质 矿化 mouse CFTCR enamel mineralization
分类号 R780.2 [医药卫生—口腔医学]
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参考文献16

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同被引文献36

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引证文献1

牙体牙髓牙周病学杂志
2003年 第6期

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