人牙胚Shh的基因克隆和序列分析

Cloning and sequenceing analysis of Shh

目的 :从人牙胚组织克隆Shh的全部编码区 ,测定并分析其基因序列。方法 :利用RT -PCR方法 ,扩增人Shh基因片段 ,将基因片段插入 pMD18-T载体 ,限制性内切酶酶切鉴定 ,测定序列。 结果 :从人牙胚中成功扩增Shh ,Blast分析与GeneBank公布的Shh基因编码区一致。结论

AIM:To clone the complete encoded sequence hu man Shh gene from human tooth germ, sequence and analyze its sequence.M ETHODS:The complete encoded sequence human Shh had been amplified by re verse transcription PCR from human tooth germ, the amplified fragment was clined into pMD18-T vector and identified by digestion and the positive clone was sequ enced by Shenggong Corporation.RESULTS:cDNA of Shh was obtained from human tooth germ ,nucle otide homology analysis indicated the sequence was identical to that of Genebank . CONCLUSION: Shh may have some effects on human tooth devel opment.