双因子对体外培养大鼠破骨细胞的诱导作用

The effect of compound cytokine on the formation of osteoclasts in vitro

目的 :研究两种诱导因子复合作用对大鼠破骨细胞体外形成的影响。方法 :采用大鼠脾细胞和骨细胞联合培养 ,实验组分别加入 1,2 5(OH) 2 D3 +M -CSF、1,2 5(OH) 2 D3 +PTH、1,2 5(OH ) 2 D3 +PGE2 、1,2 5(OH ) 2 D3 +IL -1β ,并设 1,2 5(OH) 2 D3 单因子对照组和空白对照组 ,采用TRAP染色、扫描电镜等方法观察破骨细胞形成情况。结果 :第 5d实验组单核细胞出现融合趋势 ,TRAP染色阳性 ,以 1,2 5(OH) 2 D3 +M -CSF和 1,2 5(OH) 2 D3 +PTH组破骨细胞形成数量最多。结论 :双因子联用诱导体外大鼠破骨细胞形成的作用优于单因子。

AIM:To investigate the effect of compound c yt okine on the formation of osteoclsts in vitro.METHODS:A co -culture system with rat spleen cells and osteoblastic cells was established,dif ferent bone-resorbing factors such as M-CSF,PTH,PGE 2,1L-1β were added separat ely in the cultures in the present of 1,25(OH) 2D 3,and 1,25(OH) 2D 3 was us ed as a control.TRAP staining and scan electron microscope were adopted to ident ify osteoclasts.RESULTS:After cultured for 5 d ,multinucleated cells were observed,which were positive in TRAP staining and could form absorpt ion pit on dentin slices.The group used by 1,25(OH) 2D 3 and M-CSF combined or 1,25(OH) 2D 3 and PTH combined had the best results.CONCLUSION:The effect of compound cytokine is more powerful than that of single cyt okine.