摘要
目的:探讨环氧化酶-2基因在食管上皮癌变进程中的作用及细胞周期相关基因表达的改变,为环氧化酶-2抑制剂进行食管癌化学预防提供理论依据.方法:自食管癌高发区采集食管脱落上皮细胞,对细胞涂片进行病理诊断,应用流式细胞仪对环氧化酶-2、p53、p21/WAF1及CDK4等基因在食管上皮细胞中的表达进行定量检测.结果:环氧化酶-2的表达随食管上皮细胞异型性的增高而逐渐增高,在可疑癌及癌细胞中FI=1.54±0.27,100%阳性表达.突变型p53蛋白及CDK4的表达也随细胞异型性的增高而明显增高,而抑癌基因p21/WAF1的表达则相反,其表达在重度增生及可疑癌和癌细胞中明显缺失.结论:环氧化酶-2在食管上皮癌变进程中表达升高,并同时伴有细胞周期相关基因表达异常.
AIM:To detect the expression of cyclooxygenase-2 gene and cell cycle related genes in the carcinogenesis of esophageal epithelia, so as to provide theoretical clues to the chemoprevention of esophageal carcinoma. METHODS:The samples of esophageal epithelial cells were collected from the high incidence area of esophageal carcinoma. Cell smear slides were made for the evaluation of cell differentiation. The expression of COX-2, p53, p21/WAF1 and CDK4 were detected by flow cytometry. RESULTS:The expression of COX-2 gene increased with the progression of epithelial cells from low grade dysplasia (LGD) to high grade dysplasia (HGD) or carcinoma. The fluorescence index (FI) was 1.54±0.27 in the carcinoma and suspected carcinoma groups, and the positive rate of COX-2 was 100 %. The expression of mutant p53 and CDK4 also increased as the epithelial cells progressed toward carcinoma. But the expression of tumor suppressor gene p21/WAF1 was on the opposite, and its expression sharply decreased in the HGD and carcinoma or suspected carcinoma. CONCLUSION:The expression of COX-2 gene significantly increase in the carcinogenesis of esophageal epithelia, andit also has the aberrant expression of cell cycle related genes.
出处
《世界华人消化杂志》
CAS
2003年第5期508-511,共4页
World Chinese Journal of Digestology
基金
"九五"国家攻关课题
No.96-906-01-02
河北省自然科学基金课题
No.301351~~
