摘要
目的:探讨胃癌中SMAD4/DPC4杂合性丢失(lossofheterozygosity,LOH)与胃癌临床病理的关系.方法:用多聚酶链反应-单链构相多态性(PCR-SSCP)银染法分析50例原发性胃癌SMAD4/DPC4的杂合性丢失.结果:D18S46LOH为36.2%(17/47),D18S474LOH为39.1%(18/46),DPC4LOH为59.2%(29/49).SMAD4/DPC4LOH的发生率随着胃壁浸润深度的加深而增高,随着TNM分期的增加而增高(P<0.05).胃癌直径≥5cm时,SMAD4/DPC4LOH要明显高于胃癌<5cm时(P<0.05).SMAD4/DPC4LOH在Borrmann分型中有显著性差异(P<0.05).结论:SMAD4/DPC4的杂合性丢失可能在胃癌的发展中起重要作用,是胃癌发展后期的重要分子事件.
AIM:To investigate the role of loss of heterozygosity (LOH) of SMAD4/DPC4 and the relationship between the LOH and other clinicopathological factors in gastric carcinoma. METHODS:Total 50 cases of gastric carcinoma were moni- tored with PCR-SSCP and silver staining was performed to detect LOH of SMAD4/DPC4. RESULTS:The incidence of LOH was found in 36.2 % (17/47) of D18S46, 39.1 % (18/46) of D18S474 and 59.2 % (29/49) of SMAD4/DPC4, respectively. The prevalence of SMAD4/DPC4 LOH increased progressively from intromucosal to advanced deeply invasive cancers (P <0.05). The frequency of SMAD4/ DPC4 LOH was significantly higher in TNM Ⅲ and Ⅳ stage than those in TNM Ⅰ and Ⅱ stages (P <0.05). SMAD4/DPC4 LOH was statistically significant association with tumor size (P <0.05). And a strong correlation between SMAD4/DPC4 LOH and Borrmann classification was observed (P <0.05). CONCLUSION:The SMAD4/DPC4 LOH may promote the pro- gression of gastric carcinoma and is an important molecular event in the development of the tumor at advanced stage.
出处
《世界华人消化杂志》
CAS
2003年第5期522-525,共4页
World Chinese Journal of Digestology
