黄芪对阿霉素肾病大鼠白细胞介素-2及其受体的影响

Effects of astragalus membranaceus to IL-2 and IL-2 receptor in adriamycin nephropathy

目的 :通过测定黄芪对阿霉素肾病大鼠白细胞介素 2 (IL 2 )及白细胞介素 2受体 (IL 2R)的影响 ,以探讨黄芪治疗肾病的免疫机制。 方法 :ELISA法测定血清IL 2、血清和尿可溶性IL 2R ,PAP四层法测定肾间质中表达IL 2R的细胞。 结果 :实验 4周时 ,治疗组血IL 2及尿IL 2R明显低于肾病组 (P <0 .0 5 ) ,而与正常对照组比较差异不显著 ,肾病组肾间质中表达IL 2R的细胞数明显高于正常对照组和治疗组 (P <0 .0 5 )。多因素相关分析提示 ,黄芪治疗 4周时 ,血IL 2水平与尿蛋白、血清胆固醇及三酰甘油呈正相关 (P <0 .0 5 ) ,与血清清蛋白呈负相关(P <0 .0 5 ) ;尿IL 2R与尿蛋白呈正相关 (P <0 .0 5 ) ;肾间质中表达IL 2R的细胞数与血IL 2及血IL 2R呈正相关(P <0 .0 5 )。 结论 :黄芪可能通过IL 2及IL

Objectives: To elucidate the role of IL 2 and IL 2 recepter (IL 2R) in the immunopathogenesis and the effects of astragalus membranaceus to IL 2 and IL 2R in adriamycin nephropathy. Methods: Serum IL 2, serum and urine soluble IL 2R were measured by ELISA test, the cells expressing IL 2R in interstitium were detected by PAP method. Results: In the fourth week, the serum level of IL 2 and the urinary excretion of IL 2R were significantly decreased in astragalus treated group than that in the adriamycin nephropathy group, and no difference between the astragalus treated group and the control group. In a multiple factor correlation analysis, the serum level of IL 2 was positively correlated with the amount of proteinuria, the serum level of cholesterol and triglyceride in the fourth week ( P <0.05), and was negatively correlated with the serum albumin ( P <0.05). The urinary excretion of IL 2R was positively correlated with the amount of proteinuria ( P <0.05). The cells which expressed IL 2R in interstitium was positively correlated with the serum level of IL 2 and IL 2R ( P <0.05). Conclusions: IL 2 and IL 2R were involved in the immunopathogenesis in adriamycin nephropathy and astragalus membranaceus has therapeutical effects to nephropathy through IL 2 and IL 2R.