阿尔茨海默病患者海马雌激素受体α和β共表达减少

Decreased coexpression of estrogen receptor -α and -β in the hippocampus of the patient with Alzheimer disease

目的 探测雌激素受体在阿尔茨海默病 (AD)患者海马中的变化。方法 运用免疫荧光细胞化学双标和激光共聚焦扫描显微镜 ,对 10例AD患者和 10例对照组海马进行观察 ,并进行统计分析。结果 在老年对照组和AD患者组中 ,雌激素受体α(ERα)和雌激素受体β(ERβ)均主要在锥体层神经元中表达 ,而放射层和始层中稀少 ;且多分布于胞质 ,胞核较少。ERα和ERβ免疫荧光双标细胞可分为三种类型 :(1)胞质双标细胞 ;(2 )胞核双标细胞 ;(3)ERα核染色而ERβ质染色细胞。海马CA1区胞核双标神经元与ERα阳性神经元的比率或与ERβ阳性神经元的比率 ,AD组分别为2 6 %± 0 5 %和 1 7%± 0 3% ,均低于对照组 (分别为 5 0 %± 0 7%和 3 9%± 0 6 % ) ,均 (P <0 0 0 1)。结论 雌激素受体α和 β核双标细胞的减少可能在AD发病机制中起重要作用。

Objective To detect the coexpression of estrogen receptor-α and -β (ERα and ERβ) in the hippocampus of the controls and their alterations in AD hippocampus.Methods Fluorescence immunocytochemistry and confocal laser microscopy were used to examine the coexpression of ERα and ERβ in the hippocampus in 10 controls and 10 AD cases. Results ERα or ERβ fluorescence immunoreactivity was widely observed in neurons concentrated predominantly in the stratum pyramidale and sparsely in the stratum radiatum and oriens of the hippocampus both in the controls and AD cases. The labeled pyramidal neurons for ERα or ERβ were exhibited with immunoreactivity mainly in the cytoplasm and little in the nucleus. Double-label fluorescence immunocytochemistry for ERα and ERβ in the same individual cells might be divided into three types. (1) Double-cytoplasmic-staining cells; (2) Double-nuclear-staining cells; (3) ERα-nuclear-staining and ERβ-cytoplasmic-staining cells. There were significant decreases in the ratio of double-nuclear-staining neurons to ERα positive neurons (2.6%±0.5%) or to ERβ positive neurons (1.7%±0.3%) in the subfield CA1 of AD hippocampus in comparison with that of the controls (5.0%±0.7%, P<0.001; 3.9%±0.6%, P<0.001; respectively). Conclusion The decreased double-nuclear-staining cells in AD hippocampus might play an important role in process of AD pathology.