海洛因慢性依赖大鼠神经元一氧化氮合成酶变化

Study on the changes of ncNOS in chronic heroin dependence and spontaneous withdrawal in rats

目的 研究海洛因药物滥用致脑神经细胞一氧化氮合成酶(ncNOS)表达的变化及法医学鉴定意义。方法 采用ncNOS免疫组织化学SP法、ncNOS mRNA原位分子杂交及图像分析技术,观察大鼠海洛因慢性依赖和自然戒断大脑皮质、中脑导水管周围灰质和中脑腹侧被盖区神经细胞ncNOS的变化和ncNOSmRNA的表达。结果 实验组神经细胞ncNOS含量和ncNOS mRNA表达比对照组明显增加,阳性细胞数明显增多;自然戒断组较慢性依赖组改变更加明显(P<0.05)。结论 ncNOS在海洛因慢性依赖和戒断中起重要作用,脑神经细胞ncNOS免疫组织化学变化可作为海洛因药物滥用法医学鉴定的形态学依据。

Objective To study the changes and actions of neuronal constructive nitric oxide synthase(nc-NOS) in heroin drug abuse. Methods The expression of ncNOS and ncNOS mRNA in neurons of cerebral cortex,periaqueductal gray matter and the ventral tegmental area was observed by immunohistochemistry,in situ hybridization and image analysis technique after heroin dependence and spontaneous withdrawal in rats. Results The quantity of ncNOS and ncNOS mRNA rised clearly and the number of ncNOS and ncNOS mRNA positive cells increased greatly in heroin dependence and withdrawal. The changes of ncNOS and ncNOS mRNA in spontaneous withdrawal were more clear than ones of dependence. Heroin dependence and withdrawal led to alterations in ncNOS and ncNOS mRNA expression in important regions implicated in the physical tolerance and dependence. Conclusion The ncNOS plays an important role in heroin dependence and withdrawal. The ncNOS immunohistochemical changes observed in the present study might be useful for the forensic pathological diagnosis of heroin drug abuse.