左旋精氨酸在预防阿霉素大鼠蛋白尿产生中的作用机制

Does L-arginine Prevente Proteinuria Production in Adriamycin-induced Nephrosis Model in Rats

目的 观察NO合成的前体物质L -arg对预防阿霉素大鼠蛋白尿产生中的作用机制。方法 用SD大鼠行尾静脉注射ADR造成肾病模型 ,治疗组于造模同时给予L -arg灌喂 ,观察NOET -1及肾功能的变化并行病理检查。结果 光镜及电镜证实造型成功 ,两组均出现蛋白尿 ,治疗组轻于对照组。治疗组NO水平显著高于模型组 ,NO及ET -1呈负相关 ,治疗组电镜下病理改变明显轻于模型组。结论 L -arg通过促进NO的合成及抑制ET -1的产生而达到降低蛋白尿的目的。

Objective To observe effect mechanism of L-arginine (L-arg,a precurory substance of nitric oxide sythesis) in proveting proteinuria in adriamycin-induced nephrosis model of rats.Methods Nephrosis model of 16 rats was established by tail vein injetion with adriamycin,then the 16 rats were divided into two groups;model group and treatment group with L-arg administration (treated group),the levels of nitric oxide(NO),endothelin-1(ET-1),renal function,and pathological changes were observed in the two groups.Results The successful nephrosis model of rats was indentified with optical and electron microscope technique.Proteinuria was occurred in the two groups,but proteinuria degree in treated group was lighter than those of model group,however NO level was higher than that of model group,there was negatively correlation in the levels of NO and ET-1 between both groups,pathological alteration under electron microscope observation was obviously less in the treatment group than those of the model group.Conclusions On basis of these findings,author consider that the L-arg could reduce proteinuria output by promoting NO synthesis and suppress the ET-1 production.