7β-羟基胆固醇减少铁离子所致的反应性胶质增生

7β-Hydroxycholesterol Reduces the Extent of Reactive Gliosis Caused by Fe^(3+)in Rat Brain

目的 研究 7β -羟基胆固醇抗星形胶质细胞增生的作用及其意义。 方法 大鼠脑皮质注射铁离子致胶质细胞增生 ,损伤部位同时注入 7β -羟基胆固醇脂质悬液 ,用免疫组化和图像分析技术分析 7β -羟基胆固醇对胶质纤维酸性蛋白的影响。 结果 损伤周围皮质的胶质纤维酸性蛋白阳性反应细胞数降至与正常对照组相似。结论 7β -羟基胆固醇对星形胶质细胞有明显的抗增生作用 。

Objective To demonstrate anti-proliferative effect and significance of 7β-hydroxycholesterol(7β OHCH) on astrocytes.Methods Ferric chloride were given with a cortical injection rats,then immediatedly infused liposome suspension including 7β OHCH in the injury site.Glial fibrillary acidic protein(GFAP) expression in cortex was detected quantitatively by immunohistochemistry and computerized image analysis.Results The number of GFAP positive astrocytes around the injury site was decreased to baseline.Conclusions 7β-OHCH has anti-proliferative property on astrocytes,and this could facilitate the investigation on the influences of reactive gliosis on functional recovery following brain injury and other kinds of pathogenesis involving glial cell proliferation.