亚低温处理大鼠脑缺血再灌注损伤后Caspase-3mRNA的表达变化

Changes of Caspase 3mRNA Expression After Rat Focal Cerebral Ischemia/Reperfusion Injury Treated by Mild Hypothermia

目的 探讨亚低温处理大鼠脑缺血再灌注损伤后Caspase -3mRNA的表达变化及其与损伤神经细胞凋亡的关系。方法 采用大鼠局灶性脑缺血再灌注损伤模型 ,大脑中动脉阻塞 2h ,再灌注损伤 12h ,用流式细胞仪 (FCM )和半定量逆转录聚合酶链反应 (RT -PCR)技术分别检测假手术组、对照组和亚低温组的DNA断裂百分率和Caspase -3mRNA表达水平。结果 亚低温组和假手术组DNA断裂百分率和Caspase -3mRNA表达水平均明显低于对照组。结论 亚低温处理大鼠脑缺血再灌注损伤后Caspase

Objective To explore the relationship between the changes of Caspase 3mRNA expression and the injuried nervous cell apoptosis after rat focal cerebral ischemia/reperfusion injury who was then treated by mild hypothermia.Methods The middle cerebral arteries(MCA) of SD rats were occluded for 2 hours,and reperfused for 12 hours.Using FCM and semiquantitative RT-PCR technique,the DNA fragmentation rate and Caspase 3mRNA expression level were detected in the shamoperation group,the control group and the mild hypothermia group,respectively.Results The DNA fragmentaation rate and Caspase 3mRNA expression level in the sham operation group and the mild hypothermia group were obviously lower than those in the control group.Conclusions The significantly decreased level of Caspase-3mRNA expression may be related to the obviously decreased nervous cell apoptosis after rat cerebrall ischemia/reperfusion injury treated by the mild hypothemia.