外源性TGFBI抑制乳腺癌细胞生长的体内外实验

TGFBI Inhibits Proliferation of Breast Cancer Cell in vitro and in vivo

目的研究转化生长因子β诱导基因(transforming growth factor-βinduced gene,TGFBI)是否能抑制人乳腺癌细胞株(MDA-MB-231)的体内外增生。方法将外源性TGFBI稳定转染到人乳腺癌细胞株(MDA-MB-231)中,测定细胞增殖率、细胞周期、软琼脂克隆形成率及P21、P53蛋白表达变化,检测乳腺癌细胞的致瘤性。结果外源性TGFBI在人乳腺癌细胞株(MDA-MB-231)中能够稳定地高表达;外源性TGFBI可以明显地抑制乳腺癌细胞因血清生长因子刺激的增生;与转染空质粒的对照组细胞V23101比较,外源性TGFBI相对软琼脂克隆形成数减少了90.89%;TGFBI可以使人乳腺癌细胞阻滞在G1期,延缓其进入S期的时间。外源性TGFBI可以延长裸鼠肿瘤发生的潜伏期,并降低肿瘤发生率。结论 TGFBI能够抑制人乳腺癌细胞株(MDA-MB-231)的体内外增生。

Objective To explore if transforming growth factor-β induced gene(TGFBI) could inhibit the proliferation of human breast cancer cell lines MDA-MB-231 in vitro and in vivo. Methods Ectopic TGFBI was stably transfected into MDA-MB-231. Cell proliferation, cell cycle, soft agar cloning efficiency, protein expression of P21 and P53 were analyzed to test tumorigenicity of human breast cancer cells. Results Ectopic TGFBI was expressed highly and stably in MDA-MB-231 cells, with the significant decrease of cells proliferation rate. Compared with V23101 transfected with empty plasmid, ectopic TGFBI expression resulted in a significant decrease of relative soft agar colony formation number by 90.89%. Tumor cells transfected with TGFBI were arrested in the G1 phase and delayed into the S phase. Ectopic TGFBI reduced tumorigenicity by 16.99% and delayed the incubation of tumor growth. Conclusion TGFBI could inhibit the proliferation of human breast cancer cell in vitro and in vivo.