宫颈癌及癌前病变组织中microRNAs的差异表达

Differential Expression of microRNAs in Cervical Cancer and Cervical Precancerous Lesions

目的寻找与宫颈癌及宫颈癌前病变相关的microRNA。方法利用miRNA芯片,筛查宫颈癌组织、宫颈上皮内瘤变及正常宫颈组织中差异表达的miRNA,并用实时定量RT-PCR在60份宫颈组织标本中对4个miRNA进行验证。利用生物信息学对部分差异表达的miRNA的靶基因进行功能分析。结果与正常宫颈组织比较,宫颈癌及高级别宫颈病变(HSIL)中存差异表达的miRNAs,其中在宫颈癌中下调最明显的是miR-218(下调倍数为0.175),上调最明显的是miR-21(上调倍数为5.68)。实时定量RT-PCR验证结果与miRNA芯片结果基本一致。功能分析显示预测的miR-218及miR-21的靶基因与肿瘤的生长、侵袭转移有关。结论宫颈癌及癌前病变中存在异常表达的miRNA,它们在宫颈癌发生过程中可能起癌基因或抑癌基因的作用。

Objective To identify potential miRNA involved in cervical cancer and cervical precancerous lesions. Methods miRNA microarray was applied to compare the miRNA expression profile in cervical cancer, cervical precancerous lesions and normal cervical tissues.Real-time quantificative RT-PCR was used to validate the expressions for 4 miRNAs in 60 cervical tissues. Bioinformaties programs were used to analyze potential target genes and their foundation. Results Differential miRNAs were identified in cervical cancer and high-grade squamous intraepithelial lesion(HSIL). MiR-218 was most down-regulated(0.175-fold) and miR-21 was most up-regulated(5.68-fold). Meanwhile, real-time quantitative RT-PCR result accorded with miRNA microarray result. Bioinformatic analysis indicated that the target genes of miR-218 and miR-21 may be involved in cancer invasion and metastasis.Conclusion miRNAs are aberrantly expressed in human cervical cancer and cervical precancerous lesions. These miRNAs may be involved in cervical carcinogenesis as potential tumor suppressor genes or oncogen.