食管上皮癌变过程中NF-κB与hTERT的表达及其意义

NF-κB and h TERT Expression in Esophagus Epithelial Canceration and Related Significance

目的检测食管上皮癌变过程中NF-κB与h TERT蛋白表达水平,探讨其与食管鳞状细胞癌(以下简称食管癌)癌变的关系。方法应用免疫组织化学方法检测了45例食管鳞状细胞癌、21例不典型增生和24例切缘正常组织中NF-кB和h TERT蛋白的表达水平。应用SAS统计学软件分析食管癌组织中NF-κB和h TERT蛋白表达的相关性。结果 h TERT蛋白在癌组织中的表达(88.9%)显著高于不典型增生(57.1%)和切缘正常组织(12.5%)(P<0.05),h TERT蛋白表达与患者年龄、性别、淋巴结转移及肿瘤的分化程度无关(P>0.05),与食管癌的浸润深度有关(P<0.01)。NF-кB在癌组织中的表达(80.0%)明显高于不典型增生(57.1%)和切缘正常组织(20.8%)(P<0.05),与患者年龄、性别、淋巴结转移及肿瘤的分化程度无关(P>0.05),与食管癌的浸润深度有关(P<0.01)。统计学分析,食管癌组织中NF-кB与h TERT蛋白表达呈显著正相关(P<0.05)。结论食管癌组织中h TERT及NF-κB表达异常增高可能是引起食管癌变的因素之一,联合检测h TERT及NF-кB蛋白,有助于食管癌的早期诊断及预后判断。

Objective To detect the protein expression levels of NF-κB and hTERT in esophagus epithelial canceration,and explore the related significance.Methods Immunohistochemistry was applied to detect NF-κB and hTERT protein expression in esophageal squamous cell carcinoma tissues (45 cases),atypical hyperplasia tissues (21 cases) and normal tissues (24 cases).SAS software was used to analyze the correlationship between NF-κB and hTERT protein expression in esophageal cancer tissues.Results hTERT protein expression in esophageal cancer tissues (88.9％) was significantly higher than those in atypical hyperplasia tissues (57.1％) and normal tissues (12.5％) (P＜0.05).NF-κB protein expression in esophageal cancer (80.0％) was significantly higher than those in atypical hyperplasia tissues (57.1％) and normal tissues (20.8％) (P＜0.05).NF-κB and hTERT protein expression had no correlationship with patients' age,gender,lymph node metastasis or tumnor differentiation degree (P＞0.05),but NF-κB expression had correlationship with invasion depth(P＜0.01).Statistical results showed that NF-κB and hTERT protein expression rates in esophageal cancer tissues were significantly and positively correlated(P＜0.05).Conclusion Abnormal expression of NF-κB and hTERT protein may be one of the factors for esophageal canceration.The joint detection of NF-κB and hTERT protein could provide objective index for early diagnosis and prognosis of esophageal cancer.