外周血有核细胞DNA损伤检测在肺多原发癌患者诊断中的临床价值

Clinical Value of DNA Damage Detection of Peripheral Blood Mononuclear Cells in Diagnosis of Multiple Primary Lung Cancer Patients

目的本研究旨在探讨外周血单个有核细胞DNA损伤在肺多原发癌和转移癌鉴别诊断中的临床应用价值。方法应用微量全血单细胞凝胶电泳技术(彗星实验)分别检测28例转移癌、30例肺多原发癌和29例原发肺癌患者外周血单个有核细胞DNA损伤率及损伤程度。结果转移癌组外周血单个有核细胞损伤阳性率为6.29%(176/2800),显著高于肺多原发癌组3.77%(113/3000),(χ2=18.883,P=0.000);肺多原发癌组显著高于原发肺癌组2.66%(77/2900),(χ2=5.494,P=0.019)。转移癌组外周血单个有核细胞DNA损伤的尾长、尾矩和椭圆矩分别为(44.19±13.48)μm、(4.53±1.66)和(4.64±1.50),显著高于肺多原发癌组[(34.85±8.40)μm、(3.30±1.04)和(3.54±1.03)],(P=0.000);肺多原发癌组显著高于原发肺癌组[(26.25±7.64)μm、(2.20±0.98)和(2.37±0.78)],(P=0.000)。结论检测外周血单个有核细胞DNA损伤有助于肺多原发癌与转移癌的鉴别诊断。

Objective To discuss the clinical value of DNA damage detection of peripheral blood mononuclear cells in the diagnosis of multiple primary lung cancer and metastatic carcinoma. Methods By micro-whole blood single cell gel electrophoresis assay(comet experiments), we detected DNA damage rate and damage degree of peripheral blood mononuclear cells of 28 patients with pulmonary metastasis carcinoma, 30 patients with multiple primary lung cancer, and 29 patients with primary lung cancer. ResultsDNA damage rate of peripheral blood mononuclear cell in pulmonary metastatic carcinoma group was 6.29%(176/2800), which was significantly higher than 3.77%(113/3000) in multiple primary lung cancer group(χ2=18.883, P=0.000); moreover, DNA damage rate of peripheral blood mononuclear cell in multiple primary lung cancer group was also significantly higher than 2.66%(77/2900) in primary lung cancer group(χ2= 5.494, P= 0.019). DNA damage tail length, tail moment and olive moment of patients in pulmonary metastatic carcinoma group[(44.19±13.48)μm,(4.53±1.66),(4.64±1.50)] were significantly higher than those in multiple primary lung cancer group[(34.85±8.40)μm,(3.30±1.04) and(3.54±1.03)](P=0.000); moreover, DNA damage tail length, tail moment and olive moment of patients with multiple primary lung cancer group was significantly higher than those in primary lung cancer group[(26.25±7.64)μm,(2.20±0.98) and(2.37±0.78)](P=0.000). Conclusion The detection of DNA damage in peripheral blood mononuclear cells is helpful to diagnose the difference between multiple primary lung cancer and pulmonary metastatic carcinoma.