低度恶性肌纤维母细胞肉瘤中NLRP3炎性体的表达

Expression of NLRP3 Inflammasome Proteins in Low-grade Myofibroblastic Sarcoma

目的探讨低度恶性肌纤维母细胞肉瘤(low grade myofibroblastic sarcoma,LGMS)组织中NLRP3炎性体蛋白的表达与LGMS发展、转移和预后的关系及其意义。方法采用免疫组织化学法检测13例手术切除的经病理检查确诊的LGMS瘤组织和瘤旁组织中Caspase-1、ASC和NLRP3蛋白的表达,分析瘤组织中3个NLRP3炎性体蛋白表达与临床病理特征的关系,并采用Pearson相关检验检测3个NLRP3炎性体蛋白两两之间的相关性。结果瘤旁组织中Caspase-1、ASC和NLRP3蛋白表达的平均吸光度值分别为(0.40±0.01)、(0.42±0.01)和(0.37±0.02),均明显高于肌纤维母细胞瘤组织,差异有统计学意义(P<0.05);LGMS转移患者和复发患者瘤组织中Caspase-1、ASC和NLRP3蛋白表达明显低于未发生转移患者和原发肿瘤患者(P<0.05);而瘤组织中NLRP3炎性体蛋白表达与年龄、性别和原发部位是否有慢性炎性反应无关(P>0.05)。两组中Caspase-1、ASC和NLRP3蛋白表达水平两两之间均呈明显正相关(P<0.05)。结论 NLRP3炎性体相关蛋白主要在LGMS瘤旁组织中高表达,且其与LGMS转移与预后有关。

Objective To analyze the relationship of NLRP3 inflammasome proteins expression in low-grade myofibroblastic sarcoma(LGMS) tissues with the development, metastasis and prognosis of LGMS. Methods The expression of Caspase-1, apoptosis-associated speck-like protein containing a carboxy-terminal CARD(ASC) and NLRP3 proteins in 13 pairs of LGMS tissues and adjacent cancer tissues were detected using immunohistochemical method. The relationship between the expression levels of three NLRP3 inflammasome proteins in LGMS tissues and clinical characteristics were analyzed. The correlations between every two of these three NLRP3 inflammasome proteins were detected using Pearson correlation analysis. Results The average optical density of Caspase-1, ASC and NLRP3 proteins expression in adjacent cancer tissues of LGMS were(0.40±0.01),(0.42±0.01) and(0.37±0.02), respectively, which were higher than those in LGMS tissues(P<0.05); Caspase-1, ASC and NLRP3 proteins expression in cancer tissues from LGMS patients with metastasis or recurrence were lower than those without metastasis or primary LGMS(P<0.05), but three NLRP3 inflammasome proteins expression in cancer tissues were not affected by age, gender or chronic inflammation diseases in the site of cancer(P>0.05). And there was all positive correlation between every two proteins from these three NLRP3 inflammasome proteins in two groups(P<0.05). Conclusion NLRP3 inflammasome proteins are expressed mainly in adjacent cancer tissues of LGMS, which is closely related to the metastasis and prognosis of LGMS patients.