葛根素对臂丛根性撕脱后脊髓MMP-9蛋白表达的影响

Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion

目的探讨大鼠臂丛根性撕脱后脊髓基质金属蛋白酶-9(MMP-9)蛋白的表达变化以及葛根素对其表达的影响。方法成年雄性SD大鼠174只,随机分为正常组、模型组、葛根素低、中、高处理组。模型组和葛根素处理组行右侧C_5～C_7脊神经前根撕脱术,术后腹腔注射给药。Western Blot法检测C_5～C_7节段脊髓MMP-9蛋白的表达。结果与正常组比较,臂丛神经根性撕脱后1 d时MMP-9蛋白表达达高峰,3 d降至接近正常,1周后下降至较低水平。葛根素处理组脊髓组织MMP-9蛋白表达在1 d时比模型组显著降低。结论 MMP-9可能参与臂丛根性撕脱后早期损伤反应,且葛根素可能有助于减轻该种损伤。

Objective To investigate the expression changes of matrix metalloproteinase-9(MMP-9 in spinal cord after brachial plexus root avulsion and the effect of Puerarin on its expression.Methods A total of 174 adult male SD rats were randomly divided into a normal group,a model group,Puerarin low,medium and high treatment groups.The model group and the Puerarin treatment groups underwent the right C5~C7 anterior nerve root avulsion.The rats were injected intraperitoneally Puerarin every day after the operation.The expression of MMP-9 protein in C5~C7 segment of spinal cord was detected by Western Blot.Results Compared with the normal group,the expression of MMP-9 protein peaked at the 1st day after brachial plexus root avulsion,decreased near to normal at the 3rd day and decreased to a lower level after 1 week.The expression of MMP-9 protein in spinal cord tissue of Puerarin treatment group was significantly lower than the model group at the 1st day.Conclusion MMP-9 may be involved in the early injury response to brachial plexus root avulsion,and Puerarin may help to alleviate this injury.