水通道蛋白1和4在大鼠挫伤肺组织中的表达

Expression of aquaporin 1 and 4 in lung tissue of rats with contusion injury

目的探讨水通道蛋白(AQP)1和4在老年大鼠挫伤后肺组织中的表达。方法通过自由落体模型制作老年鼠肺挫伤模型,分别于伤后1,3和6 h处死,取右侧肺上叶组织测水含量,RT-PCR法测量AQP1和4的mRNA表达,Western印迹法检测蛋白AQP1和4的表达水平。结果伤后老年大鼠的肺组织水含量比明显增加,与假手术组相比,AQP1和4的mRNA和蛋白表达在术后逐渐升高,术后6 h有显著差异(P<0.05),AQP-1的蛋白表达量亦明显升高(P<0.05)。结论 AQP1和4在挫伤后的肺组织中表达升高,靶向调节二者的表达可能对于肺挫伤后肺水肿的治疗具有重要意义。

Objective To investigate the expression of aquaporin ( AQP) 1 and 4 in lung tissues of aged rats with contusion injury . Methods The aged rat pulmonary contusion model was made by free falling body method .The rats were killed after injury for 1, 3 and 6 h. Their right upper lobe of lung tissues were took out to measure the water content .The expression of mRNA of AQP 1 and 4 were measured by PT-PCR.And the expression level of protein of AQP 1 and 4 were measured by Western blot .Results After injury, the water content in aged rats’ pulmonary tissues increased significantly .Compared with the control group , the expression of mRNA and protein of AQP 1 and 4 increased gradually after operation.And after 6 h, the differences showed statistical significance (P<0.05), as well the expression level of protein of AQP1.Conclusions The expression of AQP4 and 1 in contused lung tissue could increase significantly .Targeted adjustment might have important significance for the treatment of pulmonary edema after lung contusion .