染料木素对阿尔茨海默病模型大鼠海马区神经递质含量的影响

Effects of genistein on the content of neurotransmitter of hippocampus in Alzheimer's disease rats

目的探讨染料木素对阿尔茨海默病模型大鼠学习记忆能力及海马区神经递质含量的影响。方法健康雌性SD大鼠,随机分为5组:假手术组(Sham)、卵巢切除组(Ovx)、卵巢切除+17β-雌二醇组(Ovx+Est)、卵巢切除+17β-雌二醇+染料木素1.0组(Ovx+Est+Gen1.0)、卵巢切除+17β-雌二醇+染料木素10.0组(Ovx+Est+Gen10.0)各6只,给药21 d后取大鼠脑海马分别测定乙酰胆碱(Ach)、去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)的含量。结果 Ovx组Ach、NE、DA及5-HT递质含量与Sham组比较显著下降(P<0.05);Ovx+Est组Ach、NE、DA及5-HT递质含量与Ovx组比较显著上升(P<0.05);Ovx+Est+Gen1.0组Ach、NE、DA及5-HT递质含量与Ovx+Est组相比明显下降(P<0.05),Ovx+Est+Gen10.0组变化与Ovx+Est相似,两组相比无统计学意义。结论雌激素去除降低了脑部主要中枢神经递质的含量,外源性17β-雌二醇可升高中枢神经递质的含量,小剂量染料木素可以拮抗17β-雌二醇的这种作用,高剂量的染料木素对17β-雌二醇的作用无影响。

Objective To study the effects of genistein on learn and memory and the content of neurotransmitter of hippocampus in Alzheimer's disease( AD) rats.Methods Thirty female SD rats were randomized into five groups , each group had six rats: Sham, Ovx, Ovx+Estrogen 1.0 mg/kg body weight (Ovx+Est), Ovx+Estrogen 1.0 mg/kg body weight+Genistein 1.0 mg/kg body weight (Ovx+Est+Gen1.0), Ovx+Estrogen 1.0 mg/kg body weight+Genistein 10.0 mg/kg body weight (Ovx+Est+Gen 10.0), after administration of different estrogens for 21 d, acetylcholine (Ach), norepinephrine(NE), dopamine(DA) and 5-hydroxytryptamine(HT) were measured in hippocam-pus homogenate .Results To compare with those of Sham group , the contents of Ach ,NE,DA and 5-HT in Ovx group were decreased ( P<0.05);To compare with those of Ovx group, the contents of Ach,NE,DA and 5-HT in Ovx+Est group were increased (P<0.05);To com-pare with those of Ovx+Est group, the contents of Ach,NE,DA and 5-HT in Ovx+Est+Gen1.0 groupwere increased (P<0.05); But there was no obvious difference between Ovx+Est+Gen1.0 and Ovx+Est groups.Conclusions Estrogen removal reduces the brain's main central neurotransmitter content, after administration of exogenous 17β-estradiol, the contents of Ach,NE,DA and 5-HT are increased.Low dose of genistein could inhibit this effect of 17β-estradiol.High dose of genistein has no inhibitory effect on 17β-estradiol.