人IFN-γ基因启动子的克隆鉴定及功能分析

Identification and characterization of human IFN-γ promoter

目的克隆鉴定人IFN-γ基因启动子序列,并进行功能分析。方法通过生物信息学方法预测启动子区域,PCR克隆至pGL3报告载体,双荧光素酶法检测启动子活性。结果克隆得到人IFN-γ基因翻译起始点上游700 bp启动子区域,经检测具有活性,可启动报告基因表达;同时,该区域DNA被甲基化修饰后启动活性下降达4倍。结论克隆构建人IFN-γ基因启动子成功。

Objective To obtain and identify human IFN-γpromoter region.Methods Promoter region was predicted by bioinfor-maticsmethods,andthenclonedtopGL3reportervector.Promoteractivitywasdeterminedbydual-luciferaseassay.Results 700bpIFN-γpromoter region was obtain by PCR and had promoter activity under activation.Conclusions Vector containing human IFN-γpromoter re-gion is constructed successly.