缺氧对肺动脉血管内皮细胞线粒体膜电位的影响及Ghrelin的保护作用

The effects of hypoxia on mitochondrial membrane potential of pulmonary vascular endothelial cells and the protective effects of Ghrelin

目的观察缺氧对人肺动脉血管内皮细胞线粒体膜电位(MMP)的影响及Ghrelin的保护作用。方法将培养的人肺动脉血管内皮细胞分为对照组、单纯缺氧组和缺氧+Ghrelin组。应用激光共聚焦显微镜扫描,并测定标记的人肺动脉血管内皮细胞每秒钟MMP值;采用硝酸还原酶法和放射免疫分析技术检测人肺动脉血管内皮细胞上液中一氧化氮(NO)和内皮素(ET-1)含量。结果与对照组相比,单纯缺氧组MMP水平显著降低(P<0.05);与单纯缺氧组相比,缺氧+Ghrelin组内皮细胞MMP水平显著升高(P<0.05),缺氧+Ghrelin组与对照组内皮细胞MMP水平差异不明显(P>0.05)。单纯缺氧组上清液NO含量明显低于对照组(P<0.05),缺氧+Ghrelin组上清液NO含量明显高于单纯缺氧组(P<0.05)。单纯缺氧组上清液ET-1含量明显高于对照组(P<0.05),缺氧+Ghrelin组上清液ET-1含量明显低于单纯缺氧组(P<0.05)。结论缺氧引起人肺血管内皮细胞MMP降低,Ghrelin可抑制缺氧损伤所致MMP降低;同时Ghrelin还可以促进人肺血管内皮细胞释放NO,抑制缺氧引起的ET-1高释放。

Objective To investigate the effects of hypoxia on human pulmonary artery endothelial cells mitochondrial membrane po -tential ( MMP) , and the protective effect of Ghrelin .Methods The cultured human pulmonary artery endothelial cells were divided into con-trol, hypoxia and hypoxia +Ghrelin groups .The laser scanning confocal microscope was used to measure MMP value per second of labeled human pulmonary artery endothelial cells;nitrate reductase were measured by radioimmunoassay technique was used to detect NO and endo -thelin ( ET) -1 content in human pulmonary artery endothelial cells .Results Compared with the control group , the MMP level of hypoxia group was significantly lower (P<0.05);compared with the hypoxia group , the level of mitochondrial membrane potential of endothelial cells of hypoxia +Ghrelin group was significantly increased (P<0.05);compared with the control group, the level of mitochondrial membrane po-tential of endothelial cells of hypoxia +Ghrelin group did not change significantly (P>0.05).Supernatant NO level of hypoxia group was lower than control group(P<0.05), NO level in supernatants of hypoxia group was lower than that of hypoxia +Ghrelin group (P<0.05). ET-1 levels in the supernatant of the control group was lower than that of hypoxia group (P<0.05), that of hypoxia +Ghrelin group was low-er than that of hypoxia group(P<0.05).Conclusions Hypoxia-induced the reduced MMP in human pulmonary vascular endothelial cells could be inhibited by Ghrelin;while Ghrelin also could promote human pulmonary artery endothelial cells to release NO , inhibit high release of ET-1 induced by hypoxia .