缝隙连接蛋白-43在膀胱出口部分梗阻的大鼠逼尿肌细胞膜中的表达

Connexin-43 expressed on cell membranes in rat detrusor muscle with partial bladder outlet obustruction rats

目的探讨缝隙连接蛋白-43(Cx-43)在膀胱出口部分梗阻大鼠逼尿肌细胞膜中表达的变化。方法 33只Wistar大鼠分为模型组和假手术组,两组大鼠分别于术后2、4和8 w处死,同时进行膀胱内测压、缝隙连接数量、形态变化及Cx-43的检测。结果在模型组中缝隙连接的数量显著降低,并且术后4 w Cx-43在模型组大鼠逼尿肌细胞膜上的表达显著降低。模型组大鼠逼尿肌细胞膜上的缝隙连接数量也明显减少。结论正常的排尿过程是通过逼尿肌细胞间的缝隙连接直接作用的结果。缝隙连接介导的细胞间通讯功能障碍可能是导致排尿功能障碍的原因。

Objective To investigate alterations in gap junctional protein ,connexin-43 (Cx-43),in the rat detrusor muscle with par-tial bladder outlet obstruction (P-BOO).Methods 33 female Wistar rats (10 weeks old) were divided into model and sham groups and were killed at 2,4,and 8 weeks after surgery .Cystometric investigation ,the alteration of gap junction ,and Cx-43 protein expression ,which compose the gap junction,were examined.Results The number of gap junctions was decreased in the P-BOO rat bladder.Furthermore,de-creased cellular membrane expression of Cx-43 proteins was detected in rat detrusor muscle cells more than 4 weeks after surgery .The gap junctions of the detrusor muscle cell membranes were significantly fewer in number in the P-BOO rats with no detrusor contractions .Conclu-sions The normal signals that contribute to voiding function could be transported directly through the gap junctions .Voiding dysfunction may be caused by the disruption of gap junctional intercellular communication .