哮喘小鼠C7-T5节段脊髓后角Kinesin-1的表达

The expression of Kinesin-1 in the spinal dorsal horn of the asthmatic mice

目的探讨哮喘发病中驱动蛋白-1(Kinesin-1)在哮喘小鼠C7~T5节段脊髓后角内的表达变化。方法 BALB/c小鼠20只,按随机数字表法均分为正常对照组和哮喘组,利用AniRes2005肺功能仪测小鼠气道阻力,免疫荧光方法和Western印迹方法检测各组小鼠C7~T5节段脊髓后角内Kinesin-1的表达变化。结果哮喘组小鼠吸气阻力和呼气阻力明显高于正常组(P<0.01),哮喘模型建立成功。免疫荧光结果显示哮喘组C7~T5节段脊髓后角内Kinesin-1阳性产物的平均光密度值(MOD)显著高于正常对照组(P<0.01),Western印迹方法检测也得到了相同的结果。结论哮喘小鼠C7~T5节段脊髓后角内Kinesin-1的表达升高。

Objective To study the expression of Kinesin-1 in the spinal dorsal horn of the asthmatic mice .Methods 20 BALB/c mice were randomly divided into normal control and asthmatic groups .The airway resistance of mice was measured with AniRes 2005 pulmo-nary function meter;The expression of Kinesin-1 was observed by immunofluorescence and Western blot .Results Inspiratory resistance and expiratory resistance in the asthmatic mice were obviously higher than those of normal control mice , the asthmatic model was successfully con-structed.The mean optic density(MOD) of Kinesin-1 positive product in the C7~T5 spinal dorsal horn was increased significantly in the asthmatic mice than that in normal control by means of immunofluorescence (P<0.01), and the same result was obtained by Western blot method.Conclusions The expressions of Kinesin-1 is elevated in the C7~T5 spinal dorsal horn of the asthmatic mice .