复元胶囊对大鼠膝骨关节炎模型软骨细胞凋亡及增殖的影响

Effects of Fuyuan capsule on chondrocytes apoptosis and proliferation in rats with experimental osteoarthritis

目的观察复元胶囊对实验性大鼠膝骨关节炎(OA)模型软骨细胞凋亡及细胞核增殖抗原(PCNA)mRNA表达的影响,探讨其治疗骨关节炎的作用机制。方法 48只雄性SD大鼠随机分成正常组、模型组、壮骨关节丸组、复元胶囊组,每组12只。除正常组外,其余各组采用Hulth法建立膝OA动物模型。4 w后,壮骨关节丸组、复元胶囊组分别给与壮骨关节丸、复元胶囊灌胃,而正常组、模型组给予生理盐水。8 w后,通过肉眼观察、Mankin's评分评价复元胶囊治疗OA疗效,透射电镜、原位末端标记法(TUNEL法)检测软骨细胞凋亡,硝酸还原酶法测定关节液中一氧化氮(NO)水平,RT-PCR法检测PCNA mRNA的表达。结果复元胶囊Mankin评分、软骨细胞凋亡指数及关节液中NO水平均低于模型组(P<0.05),而PCNA mRNA的表达高于模型组(P<0.01)。结论复元胶囊能抑制OA软骨细胞的凋亡,促进软骨细胞PCNA的表达,延缓OA软骨退变过程。

Objective To observe effects of Fuyuan capsuleon chondrocytes apoptosis and expression of PCNA mRNA in rats with experimental osteoarthritis ( OA) and investigate its therapeutic mechanism on OA .Methods 48 SD male rats were divided into 4 groups randomly.OA model was induced by Hulths method .Rats in Zhuangguguanjiewan and Fuyuan capsule groups were fed with Zhuangguguan -jiewan and Fuyuan capsule , in normal and model groups were fed with 0.9%N.S.at the same time.After 8 weeks, specimens were taken. Characteristics of cartilage histological were observed by Mankin ,s method.Apoptosis of chondrocytes were determined by transmission elec-tron microscopy and TUNEL , activity of nitric oxide ( NO) in synovial fluids were measured by nitrate reductase assay and the mRNA expres -sion of PCNA was evaluated by RT-PCR.Results Fuyuan capsule could decrease Mankin ,s score, activity of NO and apoptotic cells.It could increase the mRNA expression of PCNA (P<0.05).Conclusions Fuyuan capsule could decrease apoptotic cells and increase of pro-liferation chondrocyte ,which might be related to decrease activity of No and up-regulate the mRNA expression of PCNA .