摘要
目的检测胃癌组织长链SK编码RNA(lncRNAs)的表达,寻找新的胃癌标记物和治疗靶点。方法首先使用lncRNAs表达芯片筛选胃癌组织中高表达的lncRNAs,寻找靶lncRNA。使用q PCR在较大胃癌样本中对筛选的靶lncRNA进行验证,并寻找靶lncRNA与胃癌临床特征的相关性。结果胃癌lncRNAs芯片检测显示PVT1在胃癌组织高表达。使用q PCR进行临床较大样本检测发现:PVT1在胃癌组织表达明显高于癌旁组织,PVT1在胃癌组织中高表达与淋巴结转移相关(P<0.05)。结论 PVT1在胃癌组织中高表达,可以作为新的胃癌标记物,有希望成为胃癌治疗新的靶点。
Objective To screen and identify new biomarkers for human gastric cancer from lncRNAs. Methods Expression of lncRNAs in human gastric cancer tissues was detected by lncRNAs microassays. Expression of PVT1 which was screened to have higher expression in gastric cancer tissue by lncRNAs microassays was measured by q PCR in more biopsies of human gastric cancer. Results PVT1 had higher expression in human gastric cancer tissues. Expression of PVT1 was correlated with lymph node immigration of gastric cancer. Conclusions PVT1 is a new biomarker for human gastric cancer. PVT1 has potential to become the new therapy target for treatment of gastric cancer.
出处
《中国老年学杂志》
CSCD
北大核心
2015年第6期1615-1617,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金青年基金(81300321)
福建省自然科学基金重点项目(2013J01369)
