摘要
目的探讨盐霉素对低分化鼻咽癌干细胞放疗敏感性的影响。方法将鼻咽癌干细胞悬液放入标有1~24的孔板中培养,其中1~12为对照组,13~24为实验组,其中向对照组加入200μl的PBS缓冲液,向实验组加入200μl终浓度为2μmol/L的盐霉素,适宜环境中培养12 h后,两组细胞均给予6 Gy单次照射,放疗结束后,接种至培养皿中常规培养2 w,GIEMSA染色,计算细胞存活分数(SF),采用RT-PCR及Western印迹法检测选取培养24 h后人鼻咽癌干细胞中Rad51、Ku70、Ku80基因及蛋白表达水平。结果实验组培养皿中细胞SF明显低于对照组(P<0.05)。实验组培养皿鼻咽癌干细胞Rad51、Ku70和Ku80 mRNA表达水平低于对照组(P<0.05)。实验组培养皿鼻咽癌干细胞Rad51、Ku70和Ku80蛋白表达水平明显低于对照组(P<0.05)。结论盐霉素能够明显抑制人低分化鼻咽癌干细胞中Rad51、Ku70和Ku80表达,降低细胞存活分数,提高放疗敏感性和临床疗效。
Objective To investigate the effects of salinomycin on radiation sensitivity of low differentiation nasopharyngeal carcinoma stem cells. Methods Nasopharyngeal carcinoma stem cell suspension were put into orifice plate marked with 1 ~ 24,plated with 1 ~ 12 were selected as the control group,with 13 ~ 24 were selected as the experimental group. 200 μl PBS were added into control group,200 μl salinomycin( concentration was 2 μmol / L) were added into experimental group. Cells were cultured into suitable environment for 12 h,and were given 6 Gy single radiation therapy. After radiation therapy,cells were cultured into petri dish for 2 weeks. Cell survival fraction( SF)was calculated by GIEMSA staining,Rad51,Ku70,Ku80 gene and protein expression were detected by RT-PCR and Western blot after cultured 24 h. Results SF in experimental group was lower than that of control group( P < 0. 05). Expression of Rad51,Ku70,Ku80 mRNA in experimental group were lower than those of control group( P < 0. 05). Expression of Rad51,Ku70,Ku80 protein in experimental group were lower than those of control group( P < 0. 05). Conclusions Salinomycin could significantly inhibit the expressions of Rad51,Ku70 and Ku80 in low differentiation nasopharyngeal carcinoma cells,reduce the SF,improve radiation sensitivity and clinical curative effect.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2015年第10期2601-2603,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目(No.81171365)
关键词
盐霉素
鼻咽癌
放疗
敏感性
Salinomycin
Nasopharyngeal carcinoma
Radiation therapy
Sensitivity