摘要
目的探讨重组人白细胞介素(IL)-6对胶质瘤细胞U87增生、侵袭能力的影响及其机制。方法应用CCK-8法检测IL-6对U87细胞增生的影响并计算增殖率;应用Transwell小室检测IL-6对U87侵袭的影响;应用Western印迹检测IL-6对胶质瘤细胞STAT3蛋白及其磷酸化状态的影响。结果 IL-6能促进U87增生和侵袭能力(P<0.05),并伴随STAT3及p-STAT3蛋白表达上调(P<0.05)。结论 IL-6通过促进胶质瘤细胞STAT3磷酸化,从而促进了胶质瘤细胞的增生侵袭能力,为脑胶质瘤的临床治疗提供了理论支持。
Objective To study the effect of recombinant human interleukin( IL)-6 on proliferation and invasion of Human U87 glioma cells and its mechanism. Methods CCK-8 assay was used to detect the effect of IL-6 on proliferation of U87 glioma cells,and the relative proliferation effect was calculated. Transwell assay was conducted to study the effect of IL-6 on invasion of U87 glioma cells. Western blot was performed to investigate the change of Stat3 protein and its phosphorylation status after IL-6 treatment. Results The IL-6 promoted the proliferation of U87 glioma cells( P < 0. 05). The invasion abilities of U87 cells were significantly promoted by IL-6 treatment( P < 0. 05),which was accompanied by the up-regulation of the STAT3 and p-STAT3 protein expressions. Conclusions IL-6 could effectively promote U87 glioma cells proliferation and invasion. The phosphorylation of STAT3 might play an important role in this process.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2015年第10期2617-2619,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金项目资助项目(81160312)
