Notch配体Delta-1基因真核表达载体的构建

Construction of eukaryotic expression vector of Notch ligand Delta-1

目的利用基因工程技术克隆Delta-1结构基因,构建Delta-1基因真核表达载体。方法通过Genbank公布的Delta-1基因的序列分析,设计合适的酶切位点,将Delta-1基因定向克隆到真核表达载体pc DNA3.1中,完成Delta-1真核表达载体构建,并用生物学软件对Delta-1基因序列测定及分析。结果经酶切将Delta-1基因定向克隆到真核表达载体pc DNA3.1中,序列分析结果表明,pc DNA3.1-Delta-1基因序列与Genbank Delta-1序列相比,氨基酸编码是相同的。结论成功构建了Delta-1基因真核表达载体。

Objective To clone the Delta-1 structural gene by gene engineering technique,and construct of eukaryotic expression vector of Delta-1 gene.Methods To analyze the sequence of Delta-1 gene published in Genebank, suitable restriction enzyme cutting site was designed,Delta-1 gene was cloned into the eukaryotic expression vector pcDNA3.1,the construction of eukaryotic expression vector Delta-1 was completed,and the sequence of Delta-1 gene was analyzed by using biological software.Results Delta-1 gene was cloned into the eu-karyotic expression vector pcDNA3.1 after restriction enzyme,sequence analysis showed that pcDNA3.1-Delta-1 gene sequences compared with Genbank Delta-1 sequence,the amino acid coding was same as Genbank.Conclusions The construction of eukaryotic expression vector of Delta-1 gene is successful.