芹黄素对SAMP8小鼠学习记忆及突触可塑性的影响

Effects of apigenin on learning and memory and synaptic plicity in SAMP8 mice

目的探讨芹黄素对快速老化SAMP8小鼠学习记忆能力及突触囊泡蛋白(Syn)及生长相关蛋白43(GAP43)mRNA及蛋白表达的影响。方法 6月龄SAMP8小鼠随机分为模型组、芹黄素10、20、40 mg/kg剂量组,同龄SAMP1小鼠作为对照组,用Morris水迷宫训练测试其空间学习能力和记忆能力;用Western印迹及RT-PCR方法测定海马区Syn及GAP43蛋白及mRNA表达。结果芹黄素各剂量组的平均逃避潜伏期均缩短,穿越平台区次数增加,在目标象限的停留时间延长(与SAMP8模型组相比,P<0.05),海马区Syn、GAP43蛋白及mRNA表达均较模型组增加(P<0.05)。结论芹黄素可能通过促进海马神经突触Syn及GAP43的表达,提高突触修复及再生能力,改善小鼠的学习记忆。

Objective To explore the effect of apigenin on learning and memory and the expressions of mRNA and protein of Synatophysin( Syn) and growth associated protein 43( GAP43) in senescence-accelerated mouse prone 8( SAMP8) mice.Methods The 6-month-old SAMP8 mice were randomly divided into model,apigenin 10,20,40 mg / kg administration groups,the same age of SAMP1 mice were treated as control.The spatial learning and memory capacity of mouse was determined by Morris water maze training test.The protein and mRNA expressions of Syn and GAP43 in the hippocampus of the mice were determined by Western blot and RT-PCR method.Results The average escape latency was decreased,the residence time in the target quadrant,and the times of passing through platform were increased in apigenin treated group compared with model group( P < 0.05).The protein and mRNA expressions of Syn and GAP43 in hippocampus were increased in apigenin treated group compared with model group.Conclusions Apigenin could enhance the learning and memory capability of the SAMP8 mice,which could be due to the apigenin improving synaptic repair and regeneration of hippocampal synapses by promoting the expressions of Syn and GAP43.