阿尔茨海默病患者血浆microRNA-101a-3p的表达

The expression of plasma microRNA-101a-3p in Alzheimer disease patients

目的探讨microRNA在阿尔茨海默病(AD)患者血浆中的差异变化,以寻求一个可能作为辅助诊断AD的早期生物标志物。方法实验分为患者组和健康对照组,分别取其血浆并提取microRNA,利用microRNA基因芯片和荧光定量PCR的方法寻找有表达差异的microRNA。结果基因芯片筛选出68个AD相关的差异表达microRNAs基因(P<0.05),其中miR-101a的表达水平在AD患者中显著低于对照组(log=-5.53,P=1.19×10-5),选定为感兴趣miRNA;荧光定量PCR示:患者组中miR-101a的相对表达量为8.834×10-4±2.842×10-4,对照组为6.305×10-3±1.161×10-3。患者组表达量仅为对照组14%,两组间差异有统计学意义(F=21.76,P<0.001),结果与miRNA芯片检测结果一致。ROC示:如果以0.002 163为诊断临界值,即miR-101a相对表达量低于0.002 163提示诊断AD。结论 miR-101a可能通过多种途径影响AD的发生发展,可能作为一种辅助诊断AD的早期生物标志物。

Objective To investigate the expression of plasma microRNA ( miR)-101a-3p in Alzheimer disease ( AD) patients. Methods The subjects were divided into patient and healthy controls groups .MicroRNAs were extracted from plasma of cases and measured by gene microarray and real-time PCR.Results Gene microarray and real-time PCR showed that the expression level of miR-101a-3p in AD patients was significantly reduced compared to that of healthy people .Conclusions MiR-101 a might be used as a diagnostic biomarkers in the early AD patients .