期刊文献+

沉默LAIR-1表达对乳腺癌细胞增殖和侵袭功能的影响

下载PDF
导出
摘要 目的探讨白细胞相关免疫球蛋白样受体(LAIR)-1在乳腺癌组织中的表达及其对乳腺癌细胞增殖和侵袭能力的影响。方法采用RT-PCR方法检测乳腺癌细胞系和组织标本中LAIR-1 mRNA的表达情况。采用电穿孔转染法,将LAIR-1 siRNA转染MCF-7细胞,通过Western印迹检测LAIR-1在MCF-7细胞中的表达情况。通过四甲基偶氮唑蓝比色(MTT)增殖实验检测LAIR-1对MCF-7细胞增殖的影响。通过伤Boyden小室检测LAIR-1对MCF-7细胞侵袭能力的影响。结果同癌旁组织相比,乳腺癌组织中LAIR-1 mRNA的表达量显著降低(P<0.01);且同非转移性乳腺癌组织相比,转移性乳腺癌组织中LAIR-1 mRNA的表达量亦显著降低(P<0.01)。转染LAIR-1 siRNA可显著抑制MCF-7细胞中LAIR-1的表达。抑制LAIR-1表达后,MCF-7细胞的增殖和侵袭能力均显著升高(P<0.01)。结论 LAIR-1具有抑制乳腺癌细胞增殖和侵袭的作用,乳腺癌组织中LAIR-1的低表达可能与乳腺癌的发生发展相关。
出处 《中国老年学杂志》 CAS CSCD 北大核心 2015年第19期5413-5414,共2页 Chinese Journal of Gerontology
  • 引文网络
  • 相关文献

参考文献8

  • 1Xiaobo Wang,Jingxuan Pan,Juan Li.Cytokine-induced apoptosis inhibitor 1 inhibits the growth and proliferationof multiple myeloma[J]. Molecular Medicine Reports . 2015 (2)
  • 2Hun Sik Kim,Asmita Das,Catharina C. Gross,Yenan T. Bryceson,Eric O. Long.Synergistic Signals for Natural Cytotoxicity Are Required to Overcome Inhibition by c-Cbl Ubiquitin Ligase[J]. Immunity . 2010 (2)
  • 3Bonaccorsi Irene,Cantoni Claudia,Carrega Paolo,Oliveri Daniela,Lui Gabrielle,Conte Romana,Navarra Michele,Cavaliere Riccardo,Traggiai Elisabetta,Gattorno Marco,Martini Alberto,Mingari Maria Cristina,Moretta Alessandro,Ferlazzo Guido.The immune inhibitory receptor LAIR-1 is highly expressed by plasmacytoid dendritic cells and acts complementary with NKp44 to control IFNα production. PloS one . 2010
  • 4Xiaobin Tang,Linjie Tian,Gloria Esteso,Seung-Chul Choi,Alex,er D. Barrow,Marco Colonna,Francisco Borrego,John E. Colig.Leukocyte-Associated Ig-like Receptor-1–Deficient Mice Have an Altered Immune Cell Phenotype. J. Immunol . 2012
  • 5I. Harirchi,S. Kolahdoozan,M. Karbakhsh,N. Chegini,S. M. Mohseni,A. Montazeri,A. J. Momtahen,A. Kashefi,M. Ebrahimi.Twenty years of breast cancer in Iran: downstaging without a formal screening program. Annals of Oncology . 2011
  • 6Parkin DM,Bray F,Ferlay J,et al.Global cancer statistics, 2002. CA a Cancer Journal for Clinicians . 2005
  • 7L Meyaard.The inhibitory collagen receptor LAIR-1 (CD305). Journal of Leukocyte Biology . 2008
  • 8Yin S,Zhang J,Mao Y,et al.Vav1-phospholipase C-γ1 (Vav1-PLC-γ1)pathway initiated by T cell antigen receptor (TCRγδ)activation is required to overcome inhibition by ubiquitin ligase Cbl-b duringγδT cell cytotoxicity〔J〕. Journal of Biological Chemistry . 2013

共引文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部