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Dickkopf-1对SD大鼠血管平滑肌细胞增殖、迁移及表型转化的影响

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摘要 目的观察Dickkopf(DKK)-1对SD大鼠血管平滑肌细胞(VSMC)增殖、迁移及表型转化的影响。方法取大鼠主动脉贴块培养VSMC,不同浓度外源DKK-1分别作用于VSMC,然后采用cck8和transwell试验分别检测DKK-1对大鼠VSMC增殖及迁移的影响。RT-PCR和Western印迹分别用于检测VSMC去分化表型标志基因骨桥蛋白(OPN)mRNA和蛋白表达。结果外源性DKK-1干预后,随着作用时间延长,DKK-1浓度增大,细胞增殖率降低,迁移能力减低;还可使VSMC发生表型转化,而在这个过程中OPN mRNA及蛋白表达水平明显降低。结论外源DKK-1能抑制大鼠VSMC增殖、迁移能力,并具有剂量和时间依赖性,此外,还可使VSMC从合成型向收缩型转化。
作者 王莉萍 王俊萍
机构地区 西安交通大学附属红会医院心内科 陕西省友谊医院心内科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2015年第24期7012-7014,共3页 Chinese Journal of Gerontology
关键词 DICKKOPF-1 血管平滑肌细胞 骨桥蛋白 动脉粥样硬化
分类号 R543.5 [医药卫生—心血管疾病]
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参考文献15

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中国老年学杂志
2015年 第24期

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