TLR4、MyD88和NF-κB在大肠癌中的表达及意义

Expressions of TLR4,MyD88 and NF-κB in colorectal cancer and its clin-ical significance

目的探讨Toll样受体(TLR) 4、髓样分化因子(MyD) 88、核因子(NF)-κB在大肠癌中的表达和临床意义。方法应用免疫组织化学PV6000二步法检测70例大肠癌、36例大肠腺瘤和20例癌旁正常黏膜组织中TLR4、MyD88和NF-κB的表达。结果 TLR4、MyD88和NF-κB在大肠癌中阳性表达率分别为64. 3%,74. 3%,70%,在腺瘤中阳性表达率为13. 9%,8. 3%,27. 8%,均显著高于大肠癌癌旁组织中的10%,0. 0%,15. 0%(均P<0. 05)。大肠癌组织中三者的表达存在一定关联性(P<0. 05)。结论 TLR4、MyD88和NF-κB在大肠癌中高表达,与大肠癌的分化程度、淋巴结转移、TNM分期有关,参与大肠癌的发生发展。

Objective To investigate the expressions of Toll-like receptor( TLR) 4,myeloid differentiation factor( MyD) 88,nuclear factor kappa B( NF-κB) in colorectal cancer and its clinical significance. Methods The expressions of TLR4,MyD88 andNF-κB in 70 cases of colorectal cancer,36 cases of colorectal adenoma and 20 cases of adjacent normal mucosa were detected by im-munohistochemistry PV6000 two-step method. Results The positive rates of TLR4,MyD88 and NF-κB in colorectal cancer were64.3%,74.3% and 70% respectively. The positive rates of TLR4,MyD88 and NF-κB in colorectal adenoma were 13.9%,8.3% and27.8%;10.0%,0.0%,and 15.0% of the cancerous tissues. There was a certain correlation and consistency of the expressions of thethree.Conclusions TLR4,MyD88 and NF-κB are highly expressed in colorectal cancer,which are related to the degree of differentia-tion,lymph node metastasis and TNM staging,and involve in the development of colorectal cancer.