活血通腑方对大鼠术后腹腔粘连巨噬细胞细胞因子的影响

Effect of Huoxue Tongfu decoction on cytokines of peritoneal adhesion macrophages in rats after operation

目的探讨活血通腑方对大鼠术后腹腔粘连部位白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、精氨酸酶(Arg)-1 m RNA及IL-1、IL-6、TNF-α、IL-4、IL-10、IL-13等细胞因子的影响。方法雄性SD大鼠40只采用拙刀法建立腹腔粘连模型,分为模型组、四磨汤组、氟伐他汀组、活血通腑方组各10只,另10只为假手术组。通过粘连评分评价腹腔粘连水平,实时荧光定量聚合酶链反应(RT-PCR)检测IL-6、TNF-α、Arg-1 m RNA表达水平及酶联免疫吸附试验检测IL-1、IL-6、TNF-α、IL-4、IL-10、IL-13细胞因子含量。结果与模型组相比,四磨汤组、氟伐他汀组及活血通腑方组的粘连评分均有不同程度的减轻(P<0. 05,P<0. 01),其中活血通腑方组和四靡汤组粘连评分降低显著(P<0. 01);显著降低IL-6、TNF-α、Arg-1 m RNA表达水平及减少IL-1、IL-6、TNF-α细胞因子含量(P<0. 05);显著提高IL-4、IL-10、IL-13细胞因子含量(P<0. 05)。结论活血通腑方对大鼠术后腹腔粘连具有抑制作用,可能是通过调控巨噬细胞含量达到防治术后腹腔粘连的作用。

Objective To investigate the effect of Huoxue Tongfu decoction on IL-6,TNF-α,Arg-1 m RNA,IL-1,IL-6,TNF-α,IL-4,IL-10 and other cytokines in macrophages of postoperative intra-abdominal adhesion sites.Methods Adhesion scores were used to evaluate the level of intra-abdominal adhesions.RT-PCR was used to detect the expression levels of IL-6,TNF-αand Arg-1 m RNA.The levels of IL-1,IL-6,TNF-α,IL-4,IL-10 and IL-13 were detected by enzyme-linked immunosorbent assay.Results Huoxue Tongfu decoction could significantly reduce the score of abdominal subarachnoid adhesion.Compared with the model group,Huoxue Tongfu decoction could reduce the expressions of IL-6,TNF-α,Arg-1 m RNA and IL-1,IL-6,TNF-αcytokine content;increase IL-4,IL-10,IL-13 cytokine content.Conclusions Huoxue Tongfu decoction has inhibitory effect on abdominal adhesion in rats after operation.It may be effective by regulating the content of macrophages to prevent and cure postoperative abdominal adhesion.