胆总管扩张症患儿血清肝细胞生长因子的表达及临床意义

Serum hepatocyte growth factor of expression and clinical significance in children with congenital biliary dilatation

目的探讨血清肝细胞生长因子(HGF)在胆总管扩张症患儿术前及术后的表达情况,为临床治疗及预后的评价提供理论依据。方法选择30例胆总管扩张症患儿作为试验组,分别于术前(术前组)、术后1 d(术后1 d组)、3 d(术后3 d组)及7 d(术后7 d组)采取患儿血清并保存;根据术前有无黄疸分为术前黄疸组(13例)及术前非黄疸组(17例)。同期将30例健康体检患儿血清作为对照组;采用ELISA法检测试验组及对照组HGF血清浓度,对所得数据采用t检验或单因方差分析进行处理。结果术前组、术后1 d组及术后3 d组血清HGF浓度高于对照组,相比较差异有统计学意义[(101.85±46.14)pg/ml vs.(67.57±28.08)pg/ml,P<0.05;(186.02±83.37)pg/ml vs.(67.57±28.08)pg/ml,P<0.05;(119.30±54.83)pg/ml vs.(67.57±28.08)pg/ml,P<0.05]。术后1 d组血清HGF浓度显著高于术前组,相比较差异有统计学意义(P<0.05),术后7 d组血清HGF浓度高于对照组,相比较差异无统计学意义[(72.19±18.53)pg/ml vs.67.57±28.08)pg/ml,P>0.05]。术前黄疸组HGF水平高于术前非黄疸组,相比较差异无统计学意义[(126.54±72.76)pg/ml vs.(86.54±38.96)pg/ml,P>0.05]。结论动态监测胆总管扩张症患儿术前、术后血清HGF浓度,对于了解胆总管扩张症患儿肝功能损害可能有一定的临床应用价值,对于该病的治疗及预后的评价也可能存在一定的应用前景。

Objective To investigate the expression and clinical significance of serum hepatocyte growth factor(HGF) in congenital biliary dilatation preoperatively and postoperatively and to provide a theoretical basis for clinical treatment and prognosis evaluation. Methods 30 cases of congenital biliary dilatation were selected as the experimental group, the serum samples were collected and preserved before the operation and 1st day, 3rd day and 7th day after the operation respectively. At the same time, according to the presence of jaundice the cases were divided into preoperative jaundice group and preoperative non-jaundice group. In the same period, 30 cases of healthy physical examination were used as the control group. The serum concentration of HGF was detected with ELISA methed, ang the data were processed by t test or ANOVA. Results In preoperative group, 1 d postoperative group, 3 d postoperative group serum concentration of HGF were higher than the control group and this difference was statistically significant(P<0.05). 1 d postoperative group had a significantly higher serum concentrations of HGF than preoperative group and this difference was statistically significant(P<0.05). 7 d postoperative group serum concentrations of HGF was higher than the control group, this difference was not statistically significant(P>0.05). Preoperative jaundice group HGF level was higher than that of preoperativenon-jaundice group, however, the difference was not statistically significant(P>0.05). Conclusion Dynamic monitoring preoperative and postoperative serum HGF levels in patients may have a certain clinical application value in understanding the liver function damage and may have a certain application prospect in the treatment and prognosis of the disease.