低强度超声波体外促进成骨细胞与人工骨改建的实验研究

Research of low intensity ultrasound promote osteoblast and artificial bone healing

目的研究低强度超声波(LIUS)对体外培养的人成骨样细胞MG-63生物学特性的影响,同时观察LIUS对MG-63细胞在人工骨块上的附着的影响。方法分别利用MTT、细胞化学钙钴法检测被LIUS作用后的MG-63细胞的增殖和碱性磷酸酶(ALP)表达。采用SPSS 17.0软件进行统计学分析,各组数值以均数±标准差(sx?)表示。采用Image pro-plus 6.0软件分析ALP表达情况;利用方差分析法检验组间差异的显著性。最后用扫描电镜观察MG-63细胞在人工骨块上的附着情况。结果超声作用3、7 d,MG-63细胞的增殖活力A值明显增加,差异具有统计学意义(P<0.05);且MG-63细胞胞质ALP的平均灰度值明显高于对照组,差异具有统计学意义(P<0.05)。扫描电镜下观察到通过作用了LIUS作用后的纳米羟基磷灰石/胶原/聚乳酸(n HAC/PLA)复合材料的孔隙内部可见大量MG-63细胞附着,细胞伸出伪足明显,并向四周伸展。结论 LIUS可以促进细胞增殖、增强细胞活性并可促进人成骨样MG-63细胞在纳米羟基磷灰石上的附着。

Objective To study the effects of low intensity ultrasound(LIUS) on the biological characteristics of human osteoblast-like cells(MG-63) cultured in vitro, and the effect of LIUS on the adhesion of MG-63 cells to artificial bone. Methods The proliferation and alkaline phosphatase(ALP) expression of MG-63 cells were detected by MTT and the method of chemical calcium and cobalt. The SPSS 17.0 software was used for statistical analysis, and the numerical value of each group was expressed by sx ?. Pro-Plus Image 6 software was used to analyze the expression of ALP, and the significant difference between the groups was tested by ANOVA. Finally, the adhesion of MG-63 cells on artificial bone was observed by scanning electron microscope. Results On the 3rd day and the 7th day after management by LIPUS, proliferation activity of MG-63 cell increased obviously and the difference had statistical significance(P<0.05); The average gray value of ALP in the cytoplasm of MG-63 cells was significantly higher than that in the control group, and the difference was statistically significant(P<0.05). Under the scanning electron microscope, a large number of MG-63 cells were attached to the pores of the n HAC/PLA composite after management by LIPUS, and these cells were out of pseudopod apparently, and extended to periphery. Conclusion LIUS can promote cell proliferation and enhance cell activity, which can promote the adhesion of human osteoblast-like cells(MG-63) on nano hydroxyapatite.