SPE-UHPLC-DAD法测定植物油微量酚酸类化合物的方法研究

Determination of Phenolic Acids in Vegetable Oil by SPE-UHPLC-DAD

建立了固相萃取-高效液相色谱对植物油中9种微量酚酸类化合物进行测定的方法。从提取及净化方式、流动相组成、流动相流速以及色谱柱温度等条件优化9种酚酸类化合物的检测方法。结果表明,样品经正己烷溶解,二醇基固相萃取柱(Diol-SPE)净化,甲醇-水为流动相,梯度洗脱,流速0.3 mL/min,检测波长为280 nm时,9种酚酸类化合物的检测结果最好,该检测方法的回收率在91.35%～103.21%之间,日内及日间精密度的RSD范围分别在0.3%～0.9%和0.6%～1.1%之间。该方法快速、准确,具有较好的重复性及稳定性,适合对植物油样品中9种酚酸类化合物的检测,结果为植物油的分类及真假判定提供了参考。

This study optimized the experimental conditions including extraction and cleanup methods,column temperature,and solvent composition,flow rate of mobile phase for nine trace phenolic acids detection by high-performance liquid chromatography(HPLC)after solid-phase extraction(SPE)cleanup.The results showed that the samples were dissolved in hexane.Diol group based solid-phase extraction column(Diol-SPE)purification,methanol and water as mobile phase,gradient elution,flow rate of 0.3 mL/min,the detection wavelength was 280 nm,detection of 9 kinds of phenolic compounds.The recovery of the detection method at the range of 91.35%~103.21%.The RSD of intra-day and inter-day precision was between 0.3%~0.9%and 0.6%~1.1%,respectively.The method was rapid,accurate,reproducible and stable.It is suitable for the determination of 9 phenolic acids in oil samples,and be able to provide a theoretical basis for the classification and judge determination of oils by analyzing and comparing the types and contents of phenolic acids in 16 batches of oil.