北京市温带臭虫击倒抗性基因突变检测

Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China

目的对温带臭虫电压门控钠离子通道(vgsc)基因进行测序分析,检测抗药性相关的基因突变,为该地区的臭虫治理提供依据。方法利用DNA测序技术,获得vgsc基因片段序列,分析击倒抗性相关位点的基因型。结果与参照品系相应的序列比对,现场采集的6只温带臭虫的vgsc基因序列均存在有义突变,导致编码的电压门控钠离子通道(VGSC)的氨基酸替换,即V419L和L925I突变,突变频率为100%。结论调查的样品均携带可导致击倒抗性的VGSC在419和925两个位点的突变,表明该温带臭虫种群对拟除虫菊酯类杀虫剂具有较高水平抗性。

Objective To analyze genetic mutations,predict the level of resistance to pyrethroid insecticides in the bedbug Cimex lectularius and provide a guide for bed bug control in this region.Methods Partial sequences of vgsc gene that encodes the target of pyrethroid insecticides(voltage gated sodium channel)were obtained by DNA sequencing,and the knockdown resistance-related mutations were examined.Results The six bed bugs collected on site were found to have genetic mutations at codons 419 and 925,leading to double amino acid substitutions(V419 L and L925 I)with a frequency of100%.Conclusion All the investigated bed bugs carry kdr mutations at positions 419 and 925 of the voltage gated sodium channel,predicting that this bed bug population has a higher level of resistance to pyrethroid insecticides.