妊娠期肝内胆汁淤积症动物模型的复制

Duplicate the Model of Intrahepatic Cholestasis in Pregnant Mice

目的 :研究妊娠期肝内胆汁淤积症动物模型。方法 :取体质量 2 5～ 30 g成年小鼠 ,按雌雄 2∶1合笼 ,以有阴道栓为小鼠怀孕的第 1d ,然后取怀孕小鼠 2 0只分为 2组 ,一组为妊娠对照组 ,另一组为实验组 ,另取 1 0只正常小鼠作为正常对照组。实验组从其妊娠第 1 2d开始肌肉注射苯甲酸雌二醇 ,妊娠对照组与正常对照组均注射等容量 9.0 g/L氯化钠注射液。结果 :实验组血清中的丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、碱性磷酸酶 (ALP)、总胆汁酸 (TBA)均较妊娠对照组升高 (P <0 .0 5 )。结论

Objective:To study the model of intrahepatic cholestasis in pregnant mice.Method: The weight of these adult mice was between 25 g and 30g.There were two female and one male mice in every cage. The day that pessary was found was the first day that mice were pregnant.Twenty pregnant mice were divided into normal pregnant group and experimental group.Another ten normal female mice were used as control group.Mie in experimental group were injected estradiol benzoate from 12th day when mice were pregnant.Mice in both pregnant group and normal control group were injected equal volume of normal saline.Result:The serum levels of aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase and total biliary acid increased significantly in the experimental group,compared with those of pregnant group (P<0.05).Conclusion:the animal model of intrahepatic cholestasis in pregnant mice can be made by injecting estradiol benzoate.