软肝冲剂对免疫性肝纤维化大鼠血清TGFβ_1和Ⅰ、Ⅲ型胶原水平的影响

Effect of Ruangan Granule on the Serum Levels of TGFβ_1, Ⅰ-and Ⅲ-Collagen in Rats with Liver Fibrosis

目的 :研究软肝冲剂对免疫性肝纤维化大鼠转化生长因子 β1(TGFβ1)和Ⅰ、Ⅲ型胶原水平的影响 ,以探讨软肝冲剂促进肝细胞再生及阻止肝纤维化、肝硬化形成的作用机制。方法 :复制免疫性肝纤维化动物模型 ,采用Rennard方法检测大鼠血清TGFβ1和Ⅰ、Ⅲ型胶原水平及肝脏组织羟脯氨酸含量。结果 :模型组大鼠血清TGFβ1和Ⅰ、Ⅲ型胶原水平及肝脏组织羟脯氨酸含量明显增加 ,与正常对照组比较 ,差异有显著性 (P <0 .0 1 ) ;中药软肝冲剂和西药秋水仙碱能明显降低免疫性肝纤维化大鼠血清TGFβ1和Ⅰ、Ⅲ型胶原水平及肝脏组织羟脯氨酸含量 ,与模型组比较 ,差异有统计学意义 (P <0 .0 5或P <0 .0 1 ) ,且中药高剂量组优于中药低剂量组和西药秋水仙碱组(P <0 .0 5或P <0 .0 1 ) ;中药低剂量组与西药秋水仙碱组比较 ,差异无显著性 (P >0 .0 5 )。结论 :软肝冲剂能明显降低免疫性肝纤维化大鼠血清TGFβ1和Ⅰ、Ⅲ型胶原水平 ,以促进肝细胞再生 ,抑制肝纤维化。

Objective:To study the influence of Ruangan Granule(RGG) on the serum levels of transforming growth factor β 1( TGFβ 1), Ⅰ and Ⅲ collagen in rats with liver fibrosis,and explore its mechanism. Method: The animal model was induced by bovine serum albumin(BSA). The serum levels of TGFβ 1, Ⅰ and Ⅲ collagen and the liver content of oxyproline hydroxyproline(Hyp) were detected according to Rennard′s methods. Result: The serum levels of TGFβ 1, Ⅰ and Ⅲ collagen and the liver content of Hyp all increased significantly in model group(P<0.01).RGG and colchicines could inhibit the above mentioned effects(P<0.05 or P<0.01), and the effects of the high dosage of RGG group were better than those of the low dosage of RGG group and colchicines group(P<0.05 or P<0.01),but there was no significant difference between the low dosage of RGG group and colchicines group(P>0.05). Conclusion: RGG can decrease the serum levels of TGFβ 1, Ⅰ and Ⅲ collagen and the liver content of Hyp ,which might contribute to its effects of anti hepatic injury, protecting hepatocytes, improving liver function ,and protecting against liver fibrosis.