遗传算法优化CGXⅡ培养基提高谷氨酸棒状杆菌产L-精氨酸

Optimization of CGXII medium by genetic algorithm for increasing L-arginine production by Corynebacterium glutamicum

L-精氨酸作为一种半必需氨基酸,在食品医药等行业有着极为重要的作用。培养基作为谷氨酸棒状杆菌(Corynebacterium glutamicum)生产L-精氨酸的基础,其成分及其成分之间的配比对L-精氨酸生产影响很大。利用遗传算法,以CGXⅡ初始培养基为基础,不断优化培养基成分之间的配比,L-精氨酸的积累量从0.61g/L提高至2.37g/L,提高了288.5%。通过数据分析,发现遗传算法不断优化CGXⅡ培养基的成分比例的过程中,伴随着L-精氨酸产量的大幅提升,谷氨酸棒状杆菌的生长模式发生了改变。在进一步的代谢水平分析中,发现CGXⅡ培养基的成分优化导致了谷氨酸棒状杆菌的三羧酸循环(TCA)增强。

As a semi-essential amino acid, L-arginine plays an extremely important role in food and medicine industries. Medium is used as the basis for the production of L-arginine by Corynebacterium glutamicum during fermentation, its components and the ratio between the components had a great influence on the production of L-arginine. Based on the CGXⅡ initial medium, the ratio between the components was optimized by genetic algorithm. The accumulation of L-arginine increased from 0.61 g/L to 2.37 g/L, which increased by 288.5%. Through data analysis, it was found that the growth pattern of Corynebacterium glutamicum changed with the increase of L-arginine production in the optimization process of the components ratio of CGXⅡ medium. In a further metabolic level analysis, it was found that the optimization of the composition of the CGXⅡ medium resulted in the enhancement of tricarboxylic acid cycle(TCA) of C. glutamicum.