MnMTP1在2个桑树品种间差异表达的分析

Different Expression of MnMTP1 in Two Mulberry Varieties

作为污染区替代种植的一种经济作物,了解桑树在重金属胁迫下基因表达的变化有助于从分子生物学层面解释其生理学现象。本研究以MnMTP1为目的基因,选取镉(Cd)吸收有明显差异的2个桑品种‘仙眠早’‘、浙葫芦桑’为试验材料,RT-PCR分析MnMTP1在2个桑品种根中的相对表达量变化。生物信息学分析结果表明MnMTP1具有CDF家族的典型结构,与其他物种的MTPs同源性为63%～69%。RT-PCR结果分析表明,MnMTP1基因在桑树根中的表达量在添Cd前后出现了明显变化。水培添Cd后,MnMTP1基因的相对表达量在‘仙眠早’中显著上调而在‘浙葫芦桑’中显著下调,与2个桑品种整株Cd含量的差异表现一致。试验证明,在受到Cd胁迫后,MnMTP1基因的相对表达量与桑树吸Cd能力相关。

Mulberry is a cash crop for alternative planting in contaminated area, and understanding the changes of gene expression of mulberry under heavy metal stress is helpful to explain the physiological phenomena at the molecular biology level. MnMTP1 is selected as the target gene, two mulberry varieties(‘Xianmianzao’ and ‘Zhehulusang’) with different Cadmium(Cd) absorption abilities are used as experimental materials, RT-PCR technology is used to analyze the relative expression changes of MnMTP1 in the roots of the two varieties. The results of bioinformatics analysis showed that the MnMTP1 had typical structures of CDF family and its homology with other species was 63%-69%. The results of RT-PCR showed that the relative expression level of MnMTP1 gene in mulberry roots changed significantly before and after adding Cd. After water culture with Cd, the MnMTP1 gene was significantly up-regulated in‘Xianmianzao’and down-regulated in‘Zhehulusang’, which was consistent with the Cd absorption content of the two varieties. The experiment shows that the relative expression level of MnMTP1 gene is related to the Cd absorption ability of mulberry under Cd stress.