小滨菊组培快繁体系的初步建立

Preliminary Establishment of Rapid Propagation of Leucanthemella linearis by Tissue Culture

为大量扩繁小滨菊幼苗,离体保存核心种质,进而创新菊花优异种质,培育耐涝菊花新优品种,解决菊花忌涝问题,以多年生沼生植物小滨菊(Leucanthemella linearis)的带芽茎段为外植体,开展小滨菊组培快繁体系建立的研究。结果表明,小滨菊茎段的最佳启动培养基为MS+0.5 mg/L 6-BA+0.5 mg/LNAA,启动率达到80%;最佳继代培养基为MS+0.5 mg/L 6-BA+0.2 mg/LNAA,增殖效果良好,出芽指数平均达到9.2;最佳生根培养基为1/2MS+0.3 mg/L NAA,生根率85.72%,根系生长状态良好。小滨菊茎段诱导愈伤组织的最佳培养基为MS+0.2 mg/L 6-BA+0.5 mg/L NAA,愈伤组织分化率最高的培养基为MS+1.0 mg/L 6-BA+0.2 mg/L NAA,分化率可达70.00%。

The paper aims to propagate and conserve the key germplasms and innovate the elite germplasm of Chrysanthemums used for breeding new excellent cultivars. Experiments were carried out for the standardization of micropropagation and regeneration of Leucanthemella linearis, a helophyte perennial plant.Nodal segments were used as explants and were cultured on MS medium supplemented with different concentrations of 6-BA and NAA for shoot inductions and the best result of primary cultural medium obtained was MS medium supplemented with 6-BA and NAA at the concentration of 0.5 mg/L, respectively. The best subcultural medium found was MS medium supplemented with 6-BA+NAA at the concentration of 0.5 mg/L and 0.2 mg/L, respectively, resulting about 9.2 multiple shoots from a single shoot tip explant. For root induction and elongation, 1/2 MS medium plus NAA at the concentration of 0.3 mg/Lshowed good effect, and the rooting percentage was 85.72%. Callus was induced from nodal segment on MS medium supplemented with0.2 mg/L 6-BA and 0.5 mg/L NAA, and plants regenerated best from these calluses on MS medium supplemented with 1.0 mg/L 6-BA and 0.2 mg/L NAA, the ratio of regeneration was 70.00%.