不同水稻品种OsVDAC3及3个互作蛋白基因OsV3IP1-3表达模式分析

OsVDAC3 and 3 Interaction Protein Genes OsV3IP1-3 in Rice Cultivars: Expression Pattern Analysis

为研究OsVDAC3及互作蛋白基因OsV3IP1-3的表达模式,用生物信息学和定量PCR方法分析了水稻不育系‘粤泰A’(YTA)、保持系‘粤泰B’(YTB)和‘日本晴’(NIP)不同发育时期幼穗,四叶期根、鞘和叶中OsVDAC3和OsV3IP1-3的表达量。生物信息学分析表明在ATG上游含有21个与水稻生长发育及胁迫应答相关的顺式作用元件;RiceXPro和MSU Rice Genome Annotation Project水稻基因表达数据库分析结果表明在NIP中OsV3IP1只在幼苗表达,而OsVDAC3、OsV3IP2和OsV3IP3在整个生活周期都表达,但OsVDAC3在花药表达水平最高,OsV3IP2在生殖器官表达水平最高,OsV3IP3在嫩芽中表达量最高。定量PCR结果表明OsVDAC3和OsV3IP1-3基因在NIP、YTA和YTB 3个水稻品种中表达模式不同。OsVDAC3在YTB的1.5～4.0 cm幼穗表达水平非常高,OsV3IP1在3个品种的四叶期地上部都有非常高的表达,OsV3IP2和OsV3IP3在YTB和NIP中表达模式相似,而与YTA中差异明显。这些基因的表达模式与功能的关系有待进一步研究。

To study the expression patterns of OsVDAC3 and its interaction protein gene OsV3 IP1-3, the expression of OsVDAC3 and OsV3 IP1-3 in the inflorescences of different developmental stages and the root,sheath and leaf at the fourth-leaf stage from the rice sterile line ’Yuetai A’(YTA), the maintenance line’Yuetai B’(YTB) and ’Nipponbare’(NIP) were analyzed with bioinformatics and RT-PCR. The results of bioinformatics analysis showed that there were 21 Cis-action elements related with the growth and stress response of rice in the upstream of ATG. The analysis results of rice gene expression database from RiceXPro and MSU Rice Genome Annotation Project showed that OSV3 IP1 in Nipponbare was only expressed in the seedlings, but OsVDAC3, OSV3 IP2 and OSV3 IP3 were expressed throughout the life cycle, furthermore OsVDAC3 had the highest expression in anther, OSV3 IP2 had the highest expression in the reproductive organs, and OSV3 IP3 had the highest expression in the buds. The results of RT-PCR showed that the expression patterns of OsVDAC3 and OsV3 IP1-3 were different in NIP, YTA and YTB. The expression level of OsVDAC3 in young panicles(1.5-4.0 cm) of YTB was very high. OsV3 IP1 had very high expression at the fourth-leaf stage among the 3 cultivars,the expression patterns of OsV3 IP2 and OsV3 IP3 in YTB and NIP were similar, but were different from that in YTA. The relationship between the expression patterns and functions of these genes needs further study.