干旱胁迫下水稻转录因子表达变化

Expression Change of Transcription Factors of Rice Under Drought Stress

本研究是探索在干旱应激下水稻转录因子的变化及可能的调控机制。本研究整合GEO数据库中关于水稻耐旱研究的基因芯片数据,通过TAC软件对基因芯片数据分析筛选出差异基因,利用在线数据库DAVID 6.8和Plant TF DB筛选出相应的转录因子。敏感品种有9个转录因子表达方向一致(同时上调表达的有7个转录因子,下调的有2个转录因子),耐旱品种有34个转录因子表达方向一致(同时上调表达的有23个转录因子,下调表达的有11个转录因子)。敏感品种和耐旱品种间有8个表达方向一致的重叠转录因子。干旱胁迫会显著影响转录因子的表达,不同水稻品种中,这些转录因子的表达既有较强的品种特异性,也有部分相似性。水稻可通过诱导和抑制转录因子的表达,来提高自身抗旱能力。

To investigate the change of transcription factors’ response to drought stress in rice and their potential regulatory mechanism, the data of gene chips on drought resistance of rice from GEO database were integrated. The differential genes were screened out by analyzing the data of gene chips with TAC software.And the relative transcription factors were selected with online database DAVID 6.8 and Plant TF DB. 9 transcription factor genes(7 up-regulated and 2 down-regulated at the transcription level) in drought-susceptible varieties and 34(23 up-regulated and 11 down-regulated at the transcription level) in drought-tolerant varieties had the same direction of expression. Between drought-susceptible varieties and drought-tolerant varieties, 8 overlapping transcription factors had the same direction of expression. Drought stress affected the expression of transcription factors obviously. In different varieties, these transcription factors had strong variety specificity, and some similarity. Rice can improve the drought resistance by inducing and inhibiting the expression of transcription factors.