亲水性液相色谱串联质谱法测定红甜菜中甜菜碱含量

Determination of Betaine in Red Sugarbeet by Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry

为了建立红甜菜中甜菜碱的定量分析方法,采用改进的QuEChERS方法结合液相色谱串联质谱技术,样品经快速溶剂萃取仪以甲醇为溶剂提取,经固相萃取柱净化,采用正向亲水性液相色谱柱(Hilic),梯度洗脱,通过质谱的多反应监测(multiple reactions monitoring, MRM)正离子模式扫描进行测定,外标法定量。结果表明:甜菜碱在本方法的线性范围为0.05～5μg/mL,添加水平为4～16 mg/g时,回收率为87.7%～95.7%,方法RSD值为3.69%～8.4%,检出限为0.01 mg/L,定量限为0.04 mg/L,与传统的分析方法相比,具有检出限低、灵敏度高的优点,完全可以适用于甜菜中甜菜碱的分析。

The study aims to establish a quantitative analysis method for detecting betaine in red sugarbeet.By using the modified QuEChERS method combined with liquid chromatography-tandem mass spectrometry,the sample was extracted with methanol through accelerated solvent extraction(ASE),and purified by solid phase extraction,detected by hydrophilic interaction liquid chromatography-tandem mass spectrometry under multiple reaction monitoring mode and quantified by the external standard method.The results showed that linearity range was 0.05-5 μg/mL in the test.When the adding level was 4-16 mg/g,the recovery was 87.7%-95.7%,and RSD was between 3.6% and 8.4%.The limit of detection(LOD) and the limit of quantification(LOQ) were 0.01 and 0.04 mg/L,respectively.The method is easy,quick,highly sensitive,and suitable for determination of betaine in sugabeet.