草菇菇渣栽培双孢蘑菇过程中的理化性状和木质纤维素分解利用研究

Physicochemical Properties and Lignocellulose Decomposition of Agaricus bisporus Compost Cultivated with Volvariella volvacea Mushroom Residue

为探究草菇菇渣培养料栽培双孢蘑菇的可行性,通过采集堆肥期和栽培期样品,进行培养料理化性质、木质纤维素含量及相关降解酶酶活、蘑菇生物学效率的测定。以废棉为主的草菇菇渣含氮量在1.8%以上,本试验配方二次发酵结束后含氮量达2.37%;在堆肥阶段,有62.03%的纤维素被利用,39.2%的木质素被利用;在发菌阶段,有29.32%的半纤维素被利用,有21.38%木质素被利用,木聚糖酶活性呈上升趋势,漆酶活性达到最高;在出菇阶段,纤维素、半纤维素、木质素均被部分利用,双孢蘑菇产量为22.48 kg/m2,生物学转化率达51.38%。以草菇菇渣为主要原料生产双孢蘑菇效果良好,相关降解酶活性变化与木质纤维素变化呈正相关。

To explore the feasibility of Agaricus bisporus cultivation with the Volvariella volvacea mushroom residue as the main raw material, the physical and chemical properties, the content of lignocellulose, the activities of related enzymes, and the biological efficiency of mushrooms were studied by collecting samples at the composting stage and cultivation stage. The nitrogen content in the residue of V. volvacea mushroom, which was mainly waste cotton, was over 1.8%. After the second fermentation, the nitrogen content of compost reached to 2.37% in this experimental recipe. At the composting stage, the degradation ratio of cellulose and lignin was 62.03% and 39.2%, respectively. At spawning stage, 29.32% hemicellulose and 21.38% lignin were used. At the same time, the xylanase activity showed an increasing trend, and the laccase activity reached the highest level. At the harvesting stage, cellulose, hemicellulose and lignin were partially utilized. The yield of A.bisporus was 22.48 kg/m^2, and the biological conversion efficiency was 51.38%. The effect of A. bisporus cultivated with V. volvacea mushroom residue is good, and the change of degradation enzyme activity is positively correlated with the change of lignocellulose.