电解铜离子浓度对循环水养殖系统杀菌效果的影响

The Concentration of Cu^(2+): Effects on Germicidal Efficacy in Recirculating Aquaculture System

旨在研究循环水养殖系统中铜离子浓度对系统净化效能的影响,笔者在循环水养殖系统中利用铜离子发生器添加铜离子,监测水质和斑马鱼健康状况,评价杀菌效果。结果表明:铜离子发生器的铜离子增加速度R^2=0.9942,稳定性较好。在24 h时,斑马鱼在铜离子浓度达到0.76 mg/L时,出现死亡情况;另外在同一浓度范围内,温度越低,斑马鱼对铜离子的耐受度越高,同一温度下,铜离子浓度越大,斑马鱼死亡速度越快。在安全浓度范围内(Cu^(2+)为0～0.23 mg/L),(26±2)℃为事宜养殖温度,并且0.14～0.226 mg/L范围内的铜离子浓度能有效地抑制细菌生长。本研究利用斑马鱼进行20天的养殖验证试验,随着铜离子浓度增加,硝酸盐、亚硝酸盐、总氨氮的变化趋势较为一致。铜离子浓度在0.1～0.2 mg/L之间,养殖温度(26±2)℃时,硝酸盐、亚硝酸盐、总氨氮、细菌总数处于平稳状态,有利于斑马鱼生长。

The paper aims to study the effects of concentration of Cu2+ in the recirculating aquaculture system on the purification efficiency of the system. Adding Cu2+ through the generator in the recirculating aquaculture system, we evaluated the germicidal efficacy by monitoring water quality and the health of zebrafish. The results showed that: the increase speed R2 of Cu2+ in the generator was 0.9942 with better stability;after 24 h,when the concentration of Cu2+ got to 0.76 mg/L, the zebrafish began to die;in the same concentration, the lower the temperature, the higher the zebrafish’s tolerance to Cu2+;in the same temperature, the zebrafish could die faster with the increasing concentration of Cu2+;in the safe concentration(the concentration of Cu2+was 0-0.23 mg/L), the suitable breeding temperature was(26±2)℃ and the Cu2+ could inhibit the bacteria effectively when the concentration was 0.14-0.226 mg/L. We make a 20-day zebrafish fishing test to verify the results, the concentration of nitrate nitrogen, nitrite nitrogen, TAN change similarly when the concentration of Cu2+ get higher;the concentration of nitrate nitrogen, nitrate nitrogen, TAN and the total number of bacteria keep in a stable state when the concentration of Cu2+ is 0.1-0.2 mg/L and the breeding temperature is(26±2)℃,and this condition is beneficial for the growth of zebrafish.